Lab Flashcards
Does isolation of asperigillus in lab specimen indicate lower resp infection?
No
Does isolation of legionella in a specimen indicate resp infection?
yes
For most BAL/ bronchial brushing you should use 1 mL of ____, but for Legionella, your should use 1mL of ____
Saline, sterile water
Process for collecting blood sample
- prep skin with 70% alcohol or chlorohexidine
- take before admin of antibiotics
- Kids based on body weight
- volume - 20 mL each site, 2 venipuncture sites, 10mL bottles each
Time to get sterile body fluids to lab (not blood)
15 minutes
transport media for most sterile body fluids and sterile sites (not blood)
Anaerobic cultures
Organisms to look for in sterile body sites/ fluids
S. aureus
coliforms
pseudomonas spp
Bacterioides spp.
clostridium spp.
other anaerobes
Candida
Process for suspicion of CVC causing bacteremia
- cut terminal 5 cm of catheter, transport immediately to lab (within 15 min), roll in agar
- take concurrent blood cultures from veni-puncture
Significant result:
- BSI in blood samples and >15 colonies of same organism cultured from catheter tip
What neutrophil count is consistent with bacterial peritonitis?
> 250 polymorphonuclear neutrophils
Transport time CSF
<15 minutes at RT
transport time for hair/skin/nail scrapings
within 1 day
Which surveillance definition determines if the BSI is from a central line?
Central Line Related Blood Stream Infection
Method: same organism isolated from blood cultures and catheter tip @ <10^3 CFY on the cath segment
Which surveillance def determines BSI that developed within 48 hours of insertion of a CVC or umbilical catheter?
CLABSI
General rule of thumb for positive CLABSI results
-Contamination <3%
- 2 positive cultures with commensal within 2 days
What organisms commonly contaminate blood cultures?
S. epidermindis
Bacillus spp.
Propionbacterium
S. viridans
