Path) Intro To Cellular Pathology

Question 1

Think of some special stains, what are they for. Pto for hunts

Answer 1

DPAS
EVG
Masson Fontana
Perl’s
Giemsa

Question 2

What stain is used for mucin, elastin, fibrosis, melanin, iron, mast cells

Answer 2

—

Question 3

What fixation is used? Any exceptions

Answer 3

—

Question 4

Image - endometrial adenocarcinoma / fibroids

Answer 4

—

Question 5

How to tell if there is neoplasia

What signs to look for

Answer 5

Architecture is wrong — cells don’t look normal

BIG nuclei (increased N:C ratio)
Irregular nuclear origin
Irregular distribution and texture of chromatin — iTS VISINLE

Also — atypical mitosis, hyperchomasia

Question 6

Immunohistochemistry and pathology — what is done here. What types

Answer 6

Specific proteins are identified using monoclonal antibodies.

Direct and indirect. Label and secondary antibody with enzyme

Question 7

What are the six tissues of origin and what can they consist of

Answer 7

Epithelium
Connective 
Muscle
Neural tissue
Germ line cells 
Blood

There are some markers too idk

Question 8

Cytokeratins

How can they be expressed differently

Answer 8

CK 20 or 7

CK positive or negative

Question 9

What does AE1/3 positive mean
what does CK7 positive mean
CK20 negative?
RCC positive?
PAX-8 positive?

Answer 9

—

Question 10

Codes for odd cells / cytokeratin stuff:
Meta stir carcinoma
Breast / ovary / lung / endo / thyroid 
Something interesting 
Kidney

Answer 10

—