Parasitology Flashcards
All information that was taught to me while attending Vanier College's "Animal Health Technology" Program, located in St-Laurent Montreal.
What is the generic name of pyran
Pyrantel pamoate
What is the generic name for s-125
Sulfadimethoxine
Between the modified knots technique and the difil test, which technique allows for differentiation Dirofilaria Immitis and Acanthochocheilonema reconditum microfilariae
Modified knotts technique
Why is it important to differentiate between D. Immitis and A. Reconditum ?
Because A. Reconditum doesn’t need to be treated
What are the morphological differences between both types of microfilariae
D. Immitis has a straight body with a tapered end A. Reconditum has a curved body with a blunt head and a curved tail
Can a dog have heartworm and have a negative difil or knotts test ?
Yes if he has only adult male worms or the females could not have made microfilariae yet
Give two reasons why a dog might have heartworm and yet the rest Would come out negative
Due to the fact that it tests for females. If there is too little females or only males you might get a false negative
Give the kingdom, phylum and class of dipylidium caninum ova
Kingdom: Anamalia Phylum: Platyhelminthes Class: Cestoda
What is the the common name of dipylidium caninum ova
Flea tapeworm
Describe the dipylidium caninum ova
Oval with multiple circles inside. Transparent. No visible shell
Give the kingdom, phylum and class of echinococcus granulosis cyst
Kingdom: AnamaliaPhylum: Platyhelminthes Class: Cestoda
What is the common name of echinococcus granulosus cyst
Dog worms
What are the general characteristics of echinococcus granulosus cysts
Rounded/ovalish cyst with small circle/oval circles inside
List the materials required for a fecal centrifugation technique
Slide, tongue depressor, paper cups, sample, sheathers solution
What is the density of sheathers solution
1200 units
Describe the fecal centrifugation technique
Put 1g of feces in a paper cup, put 1cc of sheathers solution in a cup. Make a soup. Put 8cc of sheathers solution. Mix it, in a second cup put 2 layers of gauze over the second cup and filter solution. Squeeze out extra liquid from gauze. Put all garbage in garbage cup
What type of centrifuge is ideal to perform fecal centrifugation
Swinging, the triac allows the coverslip to be placed on it.
Whats the difference between single and double centrifugation technique ? What is the purpose of doing the double centrifugation ?
Double centrifugation the 1st step is done with water instead of sheathers or znSO4. When you centrifuge it, te water is less dense than eggs so any sample will go to the bottom of the container. Centrifuge again, this time with sheathers and sample will raise to top
What are the materials required for a simple fecal flotation
Fecal cup, tongue depressor, zinc sulfate, cover slip, slide
Describe how to do a simple fecal flotation
Fill fecal tunnel, put zinc sulfate until divot is filled, turn until it forms a brown solution. Clip it down when the solution is nicely mixed. Full up to the top with zinc sulfate, make a miniscus, and place coverslip on top. Wait 10 mins and place it on slide
What are the disadvantages to using a direct smear
If you have a small amount of feces you can have a false negative.
What does a hydrometer measure
The specific gravity of a liquid
What does the Refractometer measure
The amount of dissolved Solutes in a solution
Which of the two should be used to measure specific gravity an check that your solutions are still good
The hydrometer
What is the kingdom, phylum and class of ancylostoma caninum ova
Kingdom: animaliaPhylum: Nematoda Class: secementea
What are the general characteristics of ancylostoma caninum ova
Oval, transparent, granular appearance
What is the common name of ancylostoma caninum ova
Hookworm
What is the kingdom, phylum and class of trichinella spiralis encysted
Kingdom: animaliaPhylum: Nematoda Class: adenophorea
What is the common name for trichinella spiralis encysted
Pork worm
What are the general characteristics of trichinella spiralis
Transparent worm in a muscle. Spiral shaped.
What is the kingdom, phylum and class of trichuris vulpis
Kingdom: animaliaPhylum: nemathelminthesClass: Nematoda
What are the general characteristics of trichuris vulpis
Oval shaped, thick outer shell with bipolar plugs
What is the common name for balis ascaris
Roundworm
What is the kingdom, phylum, class of balis ascaris
Kingdom: animaliaPhylum: Nematoda Class: secernentea
What is the common name for w. Bankrofti
Roundworm
What is the kingdom, phylum and class of w. Bankrofti
Kingdom: animalia Phylum: Nematoda Class: secernentea
How is w. Bankrofti contracted
Mosquito
What is the common name of shistosoma mansoni
Trematode
What is the kingdom, phylum and class for shistosoma mansoni
Kingdom: animaliaPhylum: Platyhelminthes Class: Trematoda
What is the main carrier of shistosoma mansoni
Snails
What is the main carrier of balis ascaris
Raccoon
How do you differentiate between Taenia and dipylidium tapeworm
Taenia had 1 pore.
How do you do the modified knotts test
1 ml blood+ 9ml formalin. Then centrifuge at 1500, 5 min. Pour off supernatant, stain and then examine sediment.
Describe the fecal centrifugation technique
1g feces, 1cc sheathers, mix. Add 8cc sheathers. Mix. Filter one cup and pour solution in. Top it up to 13 cc. Put in centrifuge: 1500, 5 min. Top it up to make a miniscus and then put coverslip. Wait 5-10 mins
How do you perform a skin scraping
Put mineral oil on crusty region, squeeze area, scrape area, put crust and material on slide with mineral oil and chop it up. Scrape till area turns red, put it on the slide and add coverslip.
How do you perform the cellophane tape method
Take tape, sticky side down multiple times, mineral oil. Put tape on slide
Describe the cellophane tape for pinworm identification
Tongue depressor, tape. Fold tape over tongue depressor with sticky side out and press it on the butt hole. Don’t use mineral oil on the slide.
What is the class of balantidium coli trophozoites
Litostomatea
What is the class of demodex follicularis
Arachnida
What is the class of fasciola hepatica
Trematoda
What is the common name of phthirus pubis adult
Crab louse
What order of lice does phthirus pubis adult belong to and how can you tell simply by looking at it
Anoplura and it is due to the smaller head then it’s body
What does the Anoplura phthirus pubis feed on
Blood
Where does the phthirus pubis live on humans
In the pubic hair or any other course hair
What is the scientific name of the head louse
Pediculus humanus capitis
Where does the head louse live
On the hair of the head
What is the baby form of a louse
A nit, or egg.
How long does a nit take to hatch to nymph form
5-14 days
What is the order of the cimex lectularis
Hemiptera
What is the common name of cimex lectularis
Bed bug
What is the order of melophagus ovinus
Diptera
What is the common name of melophagus ovinus
Sheep ked
What species are infected with sheep ked
Sheep and goats
Where does the sheep ked live on the host
Deep into the Sheeps wool or the goats fleece
What materials are needed for the baermann technique
Champagne glass with hollow tube, mesh cloth, gauze, rubber band
What solution is use in the baermann technique
Water
Describe the baermann technique
Put 5 g of feces in gauze, close the rubber band. Place mesh in glass to hold the gauze. Fill with room temp water so larva can swim out into the glass. Let it sit for 24hrs
What is the purpose of conducting the baermann test
Larva of lungworm
How is the sample for baermann test collected
Fresh sample from a hard surface
What are three different possible solutions used for fecal shippin
SAF, formalin or 70% alcohol
What is the ratio of the volume of fece to the volume of shipping solution
1/3 feces to solution
What is the class for giardia trophozoites
Protozoa
What are small segments of tapeworms called
Proglottids
How is Taenia acquired
Ingestion of intermediate host
If you are a solution with 1260 what are you
Sheathers
What is the advantage to fecal centrifugation
More efficient at separating and its rapid.
Why do we do the baermann technique
To find lungworm larva
What is the scale to calibrate the microscope called
Stage micrometer
What is the majority of interactions of living organisms based upon
Food
What is symbiosis
When two living creatures live together
What is each member of a symbiotic relationship considered
Symbiont
Describe a predator prey symbiotic relationship
Short lived. Beneficial for one and detrimental for other.
What does phoresis mean
To carry.
What is a phoresic symbiotic relationship
One animal caries another
Describe a mutualist relationship
Both organisms benefit
Describe a commensalist relationship
Benefits one organism and has no harm to the other
Describe a parasitic relationship
The parasite lives on or with the host. Metabolic dependence of one on the other.
What is the differences between predator prey relationship and parasitism
Longer relationship. Don’t usually try to kill host.
What is parasitiasis
Presence of parasite with no clinical signs.
What is parasitosis
Presences of parasites and has a disease of parasites (clinical signs)
What is parasitism
A life relationship in which the parasite uses the host as a habitat and a food source.
What are the advantages to parasitism.
Infinite amount of food b
What is advantageous to a parasite with the movement of its host
Dispersion of the parasite
What does the host protect the parasite against
The environment, heat
How does a parasite evade the host fighting it
Attach to host cells, mutate
What are some ways parasites evade the host
Anticoagulants, opiate saliva secretion, covers self with human proteins. Forms cysts
What does the trypanosoma cause
Less milk, lose weight.
What percentage of herds and individuals are infected with trypanosoma
100% of herds and 90% of individuals
What is trypanosoma considered
A chronic, subacute problem.
What are some general things that parasites cause
Anemia, diarrhea, poor growth, malabsorption, respiratory problems, cardiovascular problems, ocular issues Dermatological issues
What can you get from uncooked beef
Tapeworm
What can you get from uncooked pork
Trichanosis
What parasites can you get from contact with cats
Toxoplasmosis
What parasite can you get from dogs
Toxocaris
What are parasites that live within the body considered
Endoparasites
What do endoparasites do
Cause an infection
What are parasites that live on the body called
Ectoparasites
What do ectoparasites
Infestation
What is an example of ectoparasites
Fleas
Give characteristics of an obligate parasite
Must use the host to survive and complete the development cycle. Can have a period outside host where transmission occurs
What is an example of a facultative parasites
S. Stercoralis
What are the characteristics of a incidental parasites
Short survival
What is an example of a hyper parasite
A tape worm in a flea
What does the parasitic load factor into
The development of the disease
What is the pre patent period
Time frame from infection with a parasite to that when the parasite can be recovered and diagnosed
What is a host
Animal providing habitat for a parasite
What are some general characteristics of parasites
Have at least one definitive host and may have one or more intermediate hosts.
What happens in a definitive host
Parasite matures to sexual and adult stage
What is an intermediate host
Larval, immature and juvenile stage of parasite.
What does toxoplasma gondii do to mice
Convinces mice to approach cats. Gets rid of olfactory receptors in mice brain so mice doesn’t sense danger
What is a paratenic host
Transport host. Form of intermediate host. No development of the parasite. Remains encysted.
What is a reservoir host
Source of infection for different species
What does a vermifuge do
Paralyzed worm to expel
What does a vermicide do
Kills worm
What is the phylum for Trematodes
Platyhelminthes
What’s the class for Trematodes
Trematoda
How do you further divide the class Trematoda
Monogenic and digenetic
What are Trematodes considered
Flukes
What is the general shape of Trematodes
Flattened dorsoventrally
What is the shape is Trematodes
Leaves
Do Trematodes have segments
No
What are monogenetic Trematodes considered
Ectoparasites
What do monogenetic Trematodes inhabit
Fish, reptiles and amphibians
How do monogenetic Trematodes attach
Gills, fins, mouth and skin
What is a digenetic Trematodes
Parasitic mammals.
Why are digenetic Trematodes considered
Endoparasites
What do schistosomas resemble
A tongue
Describe the morphology of digenetic Trematodes
Have no body cavity like cestodes
How do digenetic Trematodes attach
By a ventral sucker called acetabulum
What do the 2 blind ceca do from the digenetic Trematodes
End of digestive tract that Empties into the host by means of fluke puke
Describe the acetabulum
Tiny spines which face backwards enabling them to attach themselves
Describe the sexual reproduction of Trematodes
They’re all hermaphroditic except for schistosomas
Describe the Trematodes life cycle
Eggs, embryonate in the environment, contact with water
What happens to Trematodes when they make contact with water
Hatches and makes the motile stage.
What are the motile stage of Trematodes
Miracidium
What do the Miracidium do when hatched
Swim to a snail
What do Miracidium develop into
Sporocyst
What is a Sporocyst
Sack containing many redia
What is inside a redia
Cercariae
Why does the Cercarial stage have
Has a tail, to allow swimming
What are the three options for cercariae once he is out of the snail
Penetrate the skinAttach to vegetation and encystPenetrate a second incidental host
When the cercariae exits the snail how does it penetrate the host and cost
Swimmers itch. Duck is definitive host. does not develop further in humans.
Where does s. Mansoni normally end up
Intestine
What is an abhorrent migration
When a parasite migrates to the wrong place
What is metacercaria
When the Vegetation is ingested by definitive host
What happens when the Metacercaria is digested
Assist is digested releasing a juvenile fluke. Migrates to its preferred site and becomes an adult
What is the preferred site for most trematodes
G.I. tract
What is the one trematode that does not live in the gastrointestinal tract
Paragonimus kellicotti which lives in the lungs
Where do most schistosomes live
In the circulatory system
What is the one shistosome that doesn’t live in the circulatory system
Shistosoma mansoni which lives in GI tract
what is platynosomum fastosum
Lizard poisoning fluke
What is the first intermediate host of the platynosomum fastosum
Land snail
What is the second intermediate host of platynosomum fastosum
Lizard
Describe the organs where the lizard poisoning fluke lives
Liver, gallbladder, bile ducts
What are the symptoms of lizard poisoning fluke
Jaundice, vomiting, diarrhea, death
What is an operculum
An opening
What is nanophyetus salmincola
The salmon poisoning fluke in BC. It is the smallest fluke of domesticated animals.
What is the first intermediate host of nanophyetus salmincola
Snail
What is the second intermediate host of nanophyetus salmincola
Salmon
How do you get nanophyetus salmincola
From eating uncooked salmon.
What is the largest fluke of domesticated animals
Fasciolitis magma
Where does nanophyetus salmincola live
Small intestine
What is the first intermediate host of Alaria
Snail
What is the second intermediate host of Alaria
Frog, snake, mouse
Where does alaria live
In the intestine
What is paragonimus kellicotti
Lung fluke
What is the first intermediate host of paragonimus kellicotti
Snail
What is the second intermediate host of paragonimus kellicotti
Crayfish
How is paragonimus kellicotti diagnosed
Fecal sedimentation or flotation
What is heterobilharzia Americana
Canine schistosome. Found in the Gulf states of US. Enter via the skin.
What is the intermediate host of heterobilharzia Americana
Snail
Where is heterobilharzia Americana found in the body
In the mesenteric vein of the intestine and the portal vein.
What do heterobilharzia Americana cause
Bloody diarrhea, necrosis, initiation, anorexia
What is a miricidum
A larva that is already ready and has no hatching required
How do you diagnose heterobilharzia Americana
Fecal sedimentation
What phylum and class are cestodes in
Phylum platyhelminth, class Cestoda
What are cestodes referred to as
Tapeworm
Describe the general shape of cestodes
Flattened dorsal ventrally, ribbonlike and segmented, containing segments called proglottids
Describe the morphology of eucestodes
Has a scolex at the anterior end. Some have acetabula and Rostellum
What is a scolex
A head
What is a acetabula
Suckers
What is a Rostellum
Spiky head
If a cestode has a Rostellum what do you consider him
Armed
If I cestode does not have a rostellum what do you consider him
Unarmed
What is very special about eucestodes and how they intake food
They have no digestive track. They intake their food through the skin
What can we say about the proglottids that are close to the scolex
They are young proglottids
What can we say about the proglottids that are mid-distance from scolex
They are more mature
What can we say about the proglottids that are furthest from the scolex
They’re mature and gravid
What is cross fertilization when it comes to eucestodes
One proglottid fertilizes with another proglottid
What is self fertilization when it comes to proglottids
They fertilize themselves