Parasitology

Question 1

Amoebiasis in Intestine features

Answer 1

Flash shaped ulcers which contains Erythrophagocytosis by trophozoites

Question 2

Anchovy sauce appearance is seen in

Answer 2

Liver amoebiasis

Question 3

How many stool samples for amoebiasis

Answer 3

3 consecutive

Question 4

Medium for stool culture

Answer 4

1. Polyxenic medium- has added bacterial supplements
2. Axenic medium- pure medium

Question 5

Examples of Axenic medium

Answer 5

Diamond medium

Question 6

Examples of Polyxenic medium

Answer 6

Nelson culture
Balamuth culture
Boeck and DrBohlav medium
Craige’s medium

Question 7

Differentiate between E. histolytica vs Entamoeba coli trophozoite

Answer 7

E. histolytica has engulfed RBC, central karyosome. E. coli has no RBC, eccentric karyosome

Question 8

Differentiate between E. histolytica vs Entamoeba coli cysts

Answer 8

E. histolytica-
1-4 nuclei (cartwheel app)
Thick chromatoid body made of ribonucleoprotein
E. coli-
1-8 nuclei
thin chromatoid body

Question 9

Diagnosis of amoebiasis in liver

Answer 9

Trophozoites in pus

Question 10

Amoebiasis:
1. Infective form
2. Transmission
3. Infective dose

Answer 10

1. Mature/ Quadrinucleate cyst
2. Feco oral
3. Very less

Question 11

Organisms with small infectivity dose

Answer 11

EHEC
Shigella
Cryptosporidium
E. histolytica
Giardia

Question 12

Examples of free living amoeba

Answer 12

Naegleria fowleri
Acanthamoeba

Question 13

Naegleria fowleri
1. Causes
2. Infective form
3. Spread of disease in the body
4. Diagnosis:
5. Tx

Answer 13

1. Primary amoebic meningoencephalitis
2. Trophozoites
3. Neural
4. CSF- trophozoites, Incr PMNL, Culture in NNA medium with lawn culture of E. coli
   PCR- Gold standard
5. Ampho B

Question 14

Acanthamoeba
1. ___ course
2. Spread in the body
3. Causes
4. Diagnosis
5. Infective form
6. Mostly seen in
7. Tx

Answer 14

1. C/c
2. Blood
3. Granulomatous amoebic encephalitis
4. Brain biopsy- shows trophzoites and cysts
5. Trophzoites and cysts
6. Immunodeficient people
7. Pentamidine + Sulfonamide + Azole + Flucytosine

Question 15

Similarities and differences between Balamuthia and acanthamoeba

Answer 15

Causes GAME. Shows cysts and trophozoites
Diff: FIsh shaped trophozoites, In all people (not just immunodef)

Question 16

Balantidium coli

Answer 16

1. Cysts and trophozoites have 2 nucleus each- one big kidney shaped, one small
2. In large intestine
3. Ciliated cyst
4. Largest member of amoeba family

Question 17

Examples of flagellates

Answer 17

Giardia lamblia
Trichomonas vaginalis

Question 18

Giardia lamblia
1. Site
2. Route
3. Motility
4. Infective form
5. Tests
6. Treatment

Answer 18

1. Duodenum
2. Feco oral rute
3. Falling leaf
4. Cyst
5. Stool- trophozoites- 2 nuclei and 4 pairs of flagella. Pear/ Tennis racket shaped trophozoite. Cyst- 4 nuclei
   String test/ E test
   HPE- luminal organism- sickle shape
6. Tx: Metronidazole, Tinidazole

Question 19

MC parasite infection in the world

Answer 19

Giardia lamblia

Question 20

MC parasite found in stools

Answer 20

Giardia

Question 21

MCC of STD, NGU

Answer 21

Trichomonas