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Microbiology > Bacteriology- Nocardia, Actinomyces, Mycobacterium > Flashcards

Bacteriology- Nocardia, Actinomyces, Mycobacterium Flashcards

1
Q

Motility of listeria

A

Tumbling at 25 C
Non motile at 37 C

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2
Q

Listeria
1. Flagella
2. Hemolysis shown
3. Infection via?
4. Characteristics
5. Diagnosis

A
  1. Peritrichous flagella
  2. Beta
  3. Cold contaminated food, dairy. Causes diarrhoea
  4. Catalase +, Camp +, Cold enrichment at 4 C
  5. ELISA
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3
Q

Neonatal infection of Listeria is called

A

Granulomatosis infanti septicum

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4
Q

Treatment of Listeria infection

A

Ampicillin
Gentamicin

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5
Q

Virulence of M. tb

A

Cord factor

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6
Q

What is the reason behind acid fastness of M. tb

A

Mycolic acid

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7
Q

What is the function of LAM in cell wall of M. tb?

A

It inhibits phagolysosome formation.
LAM can be tested in urine in cases of HIV with TB

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8
Q

Ghon’s focus vs Ghon’s complex vs Ranke’s complex

A

Ghon’s focus– Lung lesion
Ghon’s complex– Lung + Lymph node
Ranke’s complex– calcified Ghon’s complex

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9
Q

Simon’s focus

A

Apex involvement of TB

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10
Q

Simmond’s focus

A

Liver involvement of TB

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11
Q

Assman’s focus

A

Infraclavicular involvement of TB

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12
Q

Puhl’s

A

Supraclavicular involvement of TB

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13
Q

Weighert’s focus

A

Blood vessel involvement of TB

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14
Q

Rich’s focus

A

Brain involvement of TB

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15
Q

M. TB vs M. bovis

A

M. Tb is catalase, Niacin, Nitrate reduction positive. M. bovis is negative for all three
M.tb colonies are rough, buff and tough. M. bovis colonies are smooth, white and breaks easily

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16
Q

Sputum sample of M. tb is taken via

A

Modified Petroff method with 4% NaOH or further modified to NALC with 2% NaOH

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17
Q

ZN stain sensitivity
Lowenstein Jensen Media sensitivity for AFB

A

ZN: 10^4 AFB/ml
LJ media: 10-100 AFB/ml

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18
Q

Composition of LJ media

A

Malachite green
Egg
Salt
Asparagine

19
Q

Newer automated methods for TB detectiom

A
  1. Bactec MGIT- Mycobacterium Growth Indicator Tube
    It is fluorescent based- uses middlebrook 7H9 broth
  2. BacT/ alert- colorimetry based
  3. Versatrek- detects pressure changes
20
Q

Molecular typing methods for TB:

A
  1. RFLP using 1S6110 strain
  2. CBNAAT/ GeneXpert- within 2 hrs. Can detect 131 organism/ ml min
    It can also detect resistance (rpoB gene)
  3. Line probe assay
21
Q

Tests to detect latent TB

A
  1. Mantoux: 0.1 ml PPD
  2. Interferon Gamma Release Assay
    IF gamma is needed for granuloma formation. If T lymphocyte sample is exposed to CFP10, TB7.7, ESAT 6–> releases IF gamma which is measured by ELISA
22
Q

False + Mantoux test
False - Mantoux test

A
  1. BCG vaccine, non TB mycobacterium
  2. HIV, Immunodeficiency
23
Q

Classification for non TB mycobacteria

A

Runyon’s classification
Photochromogens
Scotochromogens
Non photochromogens
Rapid growers

24
Q

Photochromogens Examples

A

M. marinum
M. kansasii

25
Q

Scotochromogens examples

A

M. scrofulaceum
M. gordonae
M. szulgai

26
Q

Non photochromogens examples

A

M. avium
M. xenopi
M. ulcerans

‘AUX’

27
Q

Rapid growers examples in Runyon classification

A

M. chelonae
M. fortuitum

They grow within 7 days

28
Q

Which organism causes swimming pool granuloma/ fish tank granuloma?

A

M. marinum

p/w warty skin lesions

29
Q

Which are the tap water scotochromogens?

A

M. gordonae

30
Q

Which atypical mycobacteria causes Buruli ulcer?

A

M. ulcerans

31
Q

M. ulcerans produces an exotoxin called?

A

Mycolactone exotoxin

32
Q

What constitutes MAC?

A

M. avium, M. intracellulare

33
Q

Lady Windermere syndrome is caused by?

A

MAC

34
Q

Which atypical mycobacterium causes post surgery abscess?

A

M. chelonae

35
Q

Hansen’s bacillus is

A

M. leprae

36
Q

Features of M. leprae

A

Obligate anerobe
Obligate intracellular- on foot pad of mice or armadillo

37
Q

MC site of infection of nocardia vs actinomyces

A

Nocardia- lung. LN absent
Actinomyces- jaw. LN present

38
Q

MC extrapulmonary site of Nocardia infection

A

Brain

39
Q

Molar tooth appearance on solid media is shown by

A

Actinomyces

40
Q

Culture of Nocardia is done by

A

Paraffin bait technique

41
Q

Tx of Nocardia

A

Sulfonamides

42
Q

Tx of Actinomyces

A

Penicillin

43
Q

Splendore Hoeppli phenomenon is seen in

A

Actinomyces
Sporothrix fungi

44
Q

Which species of Actinomyces is a/w IUD users/ PID?

A

Actinomyces israellii

Microbiology (12 decks)

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