OVERVIEW OF HISTOPATHOLOGY

Question 1

what do you need to check in the labels after receiving samples?

Answer 1

Name
Age
Sex
Clinical data
Surgical findings
Nature of operation
Name of tissues submitted

Question 2

what are the serial numbers assigned in the following:
- autopsy
- surgical/biopsy
- specimen

Answer 2

A
S
SP

Question 3

why serial numbers are important

Answer 3

avoid interchanging of samples

Question 4

Performed by a pathologist; Allows you to know more about the pathologist

Answer 4

gross examination

Question 5

size of specimens to be cut in gross examination

Answer 5

2cm2 and not more than 4mm in
thickness

Question 6

macroscopic characteristics need to take note during gross examination

Answer 6

Color
Size
Texture
Consistency

Question 7

reagent used in fixation

Answer 7

formalin

Question 8

what is the use of formalin

Answer 8

preserve the sample

Question 9

Preserved in a state as close to living
tissues

Answer 9

fixation

Question 10

terminate any on-going biochemical
reactions.

Answer 10

fixatives

Question 11

protect tissues from decay

Answer 11

fixation

Question 12

reagent used in decalcification

Answer 12

nitric acid

Question 13

step that can be skipped depending on the tissue and is the removal of calcium deposits

Answer 13

decalcification

Question 14

examples of tissues that undergo decalcification

Answer 14

Bone
Teeth
Cartilage
Arteries with atherosclerotic plaque
formation

Question 15

deposition of cholesterol on the walls or the lumen of the arteries

Answer 15

Atherosclerotic plaque formation

Question 16

reagent used in dehydration

Answer 16

alcohol

Question 17

extract water in a gradual manner from the tissue specimen

Answer 17

dehydration

Question 18

reagent used in clearing

Answer 18

xylene and toluene

Question 19

A transition step between dehydration
and infiltration.

Answer 19

clearing

Question 19

true or false: clear agents are low volatile

Answer 19

false

Question 20

reagent used in infiltration

Answer 20

melted paraffin wax

Question 21

Removal of the excess clearing agent

Answer 21

inflitration

Question 22

reagent used in embedding

Answer 22

melted paraffin wax

Question 23

other name for embedding?

Answer 23

casting or blocking

Question 24

Solidification of the tissue sample to
view the structure of the sample and
facilitate ease of sectioning.

Answer 24

embedding

Question 25

cutting of the embedded tissues into
uniformly thin slices

Answer 25

cutting or sectioning

Question 26

equipment used in cutting or sectioning

Answer 26

microtome

Question 27

Process of adding colors or dyes to the
thin tissue slices for enhanced
visualization and differentiation of
cellular structures.

Answer 27

staining

Question 28

reagent used in staining

Answer 28

hematoxylin and eosin

Question 29

• Blue to purple
• Basic dye
• It will stain the acidic portions of the cell

Answer 29

hematoxylin

Question 30

• Pink to red
• Acidic dye
• Will stain the basic portions of the cell

Answer 30

Eosin

Question 31

• Stained with basic dyes
• BASOPHILIC

Answer 31

Negative charges (anionic)

Question 32

• Readily stained with acidic dyes
• ACIDOPHILIC

Answer 32

Positive charges (cationic)

Question 33

Allows the tissue to be used for longer
periods of the time since the tissue
does not decay throughout long periods
of storage.

Answer 33

mounting

Question 34

medium used in mounting

Answer 34

glycerin and resin