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histopathology > INSTRUMENTATION > Flashcards

INSTRUMENTATION Flashcards

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1
Q

components of histopathology lab

A

equipment and apparatus
wares
chemicals and reagents
dyes

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2
Q

what can you see in a gross table

A

➢ Forceps
➢ Scalpel
➢ Chopping board
➢ Weighing balance
➢ Ruler
➢ Orientation markers
➢ Watch glass/ petri dish
➢ Tissue cassettes

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3
Q

This apparatus can be automatically set
to process later the day or whenever
you want.

A

Automated tissue processor

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4
Q

we place our slides right after
sectioning

A

Paraffin oven

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5
Q

allows the fast hardening of our paraffin
wax for sectioning

A

Embedding center

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6
Q

what is the recommended temperature in floatation

A

5-10 degrees Celsius BELOW the melting
point

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7
Q

arranging the orientation of our tissue
sections on the slide.

A

fishing out method

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8
Q

remove the excess paraffin and water
on our slide right after placing it in the
floatation bath

A

Slide dryers

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9
Q

what is the temp for slide dryers

A

5-10 degrees above the melting point of
the paraffin wax

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10
Q

automatically transfers the slide from
one stain to another

A

Automated stainers

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11
Q

who uses the microscope in the histopath lab

A

pathologist

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12
Q

most expensive biopsy, for our frozen sections

A

Cryostat

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13
Q

temp for cryostat

A

-15 to -30 degrees Celsius

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14
Q

temp for paraffin was dispenser

A

2-4 degrees Celsius ABOVE the
melting point of the paraffin wax

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15
Q

place here reagents that require
refrigerator temperature for
preservation

A

Refrigerator

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16
Q

exposing heat to our tissue can speed
up the process

A

Microwave oven

17
Q

what type of microtome is used for cutting semi-thin to thin sections of paraffin wax

A

Rotary Microtome

18
Q

what type of microtome is used for

A

cutting large blocks of paraffin and resin embedded material including whole
organs

19
Q

The razor of this microtome is fixed and the specimen to be sliced for
microscopic examination passes up
and down in an arc of a circle across the
razor in a rocking motion.

A

rocking microtome

20
Q

who is the inventor of rocking microtome

A

sir horace darwin

21
Q

what type of microtome used to cutting thin to semi-thin (about 8-12μm)
sections of fresh, frozen tissue and has a temp of -15 to -30 degrees Celsius

A

Freezing Microtome

22
Q

what type of microtome is used for cutting sections of frozen tissue sections as thin as 1 micron are possible.
Temperature. (-15 to -30C) to allow the
hardening of the tissue section.

A

cryostat

23
Q

what type of microtome is used to prepare ultrathin sections for light and
electron microscopy

A

Ultrathin Microtome

24
Q

types of wares

A

molder
coplin jars
beakers
tissue cassete

25
Q

For holding substances

A

wares

26
Q

where we place our slides when we are performing staining

A

coplin jars

27
Q

Tissue casette: yellow

A

liver and renal tissue

28
Q

Tissue casette: green

A

any tissue

29
Q

Tissue casette: white

A

bone tissue

30
Q

Tissue casette: gray

A

skin biopsy

31
Q

Tissue casette: pink

A

lymph nodes

32
Q

what color of cassetes does the ph uses?

A

yellow and white

33
Q

what reagents are found in dehydration

A

70% isopropyl alcohol
80% isopropyl alcohol
95% isopropyl alcohol
Absolute alcohol-I
Absolute alcohol-II

34
Q

what reagents are found in clearing

A

Xylene-I
Xylene-II
Xylene-III

35
Q

what reagents are found in impregnation

A

Paraffin Wax-I
Paraffin Wax-II
Paraffin Wax-III

36
Q

the principal tissue stains for histology
or histopathology processes.

A

Hematoxylin and Eosin stain

37
Q

Used to screen cervical cancer

A

Papanicolaou Stain

histopathology (11 decks)

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