DEHYDRATION

Question 1

removal of fixative and water from tissue

Answer 1

dehydration

Question 2

fixative ratio of dehydration

Answer 2

1:10

Question 3

characteristics of ideal dehydrating solution

Answer 3

rapid action
does not evaporate fast
able to dehydrate fatty tissues
not harden tissues excessively
not remove stains

Question 4

it is done in an ascending grades to avoid distortion of tissue

Answer 4

alcohol

Question 5

ascending grades of alcohol

Answer 5

70% ROH
90% ROH
100% ROH

Question 6

for delicate tissues why do we need to start at 30% of alcohol?

Answer 6

to avoid the shrinkage and brittleness of tissues

Question 7

dehydration rate of alcohol

Answer 7

37 degrees celsius

Question 8

best dehydrant under alcohol because it is fast acting, mixes with water, not poisonous, cheap, clear, and flammable

Answer 8

ethanol

Question 9

it is toxic and ideal for blood and smear preparation fixation

Answer 9

methanol

Question 10

a slow-acting alcohol that is made up of plant and animan microtechnique

Answer 10

butyl alcohol

Question 11

fastest dehydrant (30 mins to 2hrs), highly flammable, evaporates fast and not recommended for routine dehydration purposes

Answer 11

acetone

Question 12

other name of dioxane

Answer 12

diethylene dioxide

Question 13

what is the purpose of dioxane

Answer 13

dehydrating and clearing agent

Question 14

wrapping tissue in gauze ans suspension to a bottle containing dioxane with anhydrous calcium oxide to quicklime

Answer 14

weiseberger’s method

Question 15

rapid, prolonged storage does not cause distortion to tissues, toxic inhalation, skin irritant and combustible at 110-120 F

Answer 15

cellosolve

Question 16

triethyl phosphate is soluble in?

Answer 16

alcohol, water, ethyl benzene, chloroform, acetone, and xylene

Question 17

disadvantage of triethyl phosphate

Answer 17

produces minimum shrinkage and not commoly available in the market

Question 18

two dehydrating agents that can act as dehydrant and clearing agent

Answer 18

dioxane
tetrahydrofuran

Question 19

additives of dehydrating agents

Answer 19

4% phenol
glycerol/alcohol mixture