CLEARING Flashcards
trans based
removing dehydrant forn the tissue and replacing it with a fluid miscible to both dehydrant and embedding agent. makes tissues transluscent. most flammable fluids have low boiling points and excessive clearing may cause brittleness
clearing
main purpose of clearing
create a bridge between the process of dehydration and infiltration
characteristics of good clearing agent
miscible with alcohol and paraffin wax
not produce tissue shrinkage
make tissue transparent
does not dissolve in aniline dyes
not evaporate quickly in water bath
time for xylene
30 mins to 1 hr
time for toluene
1hr to 2 hrs
time for benzene
15 mins to 1 hr
it is an advantage of an agent where it is rapid, makes tissues transparent, miscible in alcohol and paraffin and cheap
xylene
it is a disadvantage of an agent where it is slower than xylene and more expensive
toluene
a disadvantage of an agent where it is carcinogenic and may lead to aplastic anemia
benzene
what is carcinogenic
potential to cause a cancer
an advantage to an agent where it is not carcinogenic, miscible in both alcohol and paraffin and does not make the tissues hard
toluene
a disadvantage of an agent thatis highly flammable, makes tissues hard after for more than 3 hours and not suitable for nervous tissues and lymph nodes
xylene
an advantage of an agent where it is used for urgent biospsies and the fastest clearing agent
benzene
a clearing agent that is used for tough tissues and large specimens and is toxic to liver
chloroform
a clearing agent that is used for smooth muscle tissue and cns
cedarwood oil
a clearing agent that is used for insects, embryos, and delicate tissues
aniline oil
a clearing agent that is causes minimum shrinkage of tissues and usually slow, expensive, and difficult to use
clove oil
a clearing agent that is used for tough tissues and large specimens, cheap, and dangerous to inhale on prolonged exposure
carbon tetrachloride
a clearing agent that is nontoxic but with offensive odor and considered superior among other agents since it can process dehydration and clearing and it can save time
tetrahydrfuran
a clearing agent that causes greater shrinkage than xylene and toxic to liver
dioxane
a clearing agent that is used when clearing directly from water
gum syrup and glycerin
a clearing agent that is used for skin and smooth muscle
oil of bergamot
a clearing agent that is used for skin
oil of origanum
a clearing agent that is used for delicate tissues
oil of wintergreen
a clearing agent that is used for smooth muscle and has foul odor
carbon disulfide
a clearing agent that is used for friable tissues
carbol xylene
a clearing agent that is used for delicate tissues like eyes
terpineol
a clearing agent that is used for smooth muscles
phenol
a clearing agent that is used excellent as an clearing agent
high test aviation lead-free gasoline
