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HISTOPATHOLOGY > EMBEDDING AND INFILTRATION > Flashcards

EMBEDDING AND INFILTRATION Flashcards

trans base

1
Q

Process that removes the clearing agent

A

impregnation

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2
Q

3 types of general tissue impregnation

A

paraffin wax impregnation
celloidin impregnation
gelatin impregnation

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3
Q

range temp of actual melting wax

A

56 celcius
58 celsius

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4
Q

solid in form upon procurement thus we need to melt it first in order for us to have it in
liquid form.

A

paraffin wax

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5
Q

must be maintained 2-5°C above
the melting point of wax

A

paraffin oven

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6
Q

what coarse filter must be used in impregnation

A

Green’s No. 904

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7
Q

3 Ways of Paraffin Wax Impregnation

A

manual processing
automatic processing
vacuum embedding

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8
Q

how many changes of paraffin dp we do in manual processinh and how long per beaker/change

A

3 changes of paraffin, 1hr per beaker/change

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9
Q

how many stations are there in automatic processing and infiltration will take up at what stations?

A

12 station; 11 and 12

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10
Q

fastest way of embedding yet the most expensive

A

vacuum embedding

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11
Q

more elastic and resilient

A

Paraplast

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12
Q

Less brittle, and less compressible

A

Embeddol

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13
Q

semisynthetic; for embedding of eyes

A

Bioloid

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14
Q

melting point of paraplast

A

56-57°C

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15
Q

melting point of embeddol

A

56-57°C

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16
Q

melting point of ester wax

A

46-48°C

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17
Q

melting point of water soluble waxes (polyethylene glycol)

A

38-42 or 45-56 celsius

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18
Q

most common water soluble wac and no need for dehydration and clearing

A

Carbowax

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19
Q

remedy for carbowax

A

add soap to water or 10% PEG 900 in water

20
Q

Purified form of nitrocellulose/gun cotton

A

colloidin

21
Q

concentrations in celloidin impregnation

A

2%
4%
8%

22
Q
  • for bones, brain, teeth
    ◆ Store tissue block in 70%-80% alcohol
    ◆ The purpose of storing it in this
    concentration is to avoid dehydration and
    shrinkage of the tissue.
A

wet

23
Q
  • for whole eye sections
    ◆ Store tissue block in Gilson’s Mixture
    (chloroform and cedarwood oil)
A

dry

24
Q

Has lower viscosity, thus can be used in higher concentration, and rapid tissue penetration

A

Nitrocellulose

25
Q

Needed to prevent tissue cracking in chrome mordanted tissues

A

Plasticizer

26
Q

rarely used impregnation

A

gelatin impregnation

27
Q

must be added to prevent molds and prevent further damage such as tissue decay

A

1% phenol

28
Q

Placing the impregnated tissue into a mold with embedding media, and then allowing the media to solidify

A

embedding

29
Q

other terms for embedding

A

casting
blocking
molding

30
Q

orientation for tubular tissue

A

transverse sections

31
Q

orientation for skin

A

all layers should come

32
Q

orientation for endomatrial curetting

A

keep in center

33
Q

orientation for long tissue

A

keep diagonally

34
Q

orientation for intestine

A

all layers should come

35
Q

orientation for membrane

A

swiss roll

36
Q

types of molds

A

Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base Mold
Disposable Embeding Molds

37
Q

It has two (2) L-shaped metal plates, strips of heavy brass metal.

A

Leuckhart’s Embedding Mold

38
Q

This used for batch embedding thus one of its disadvantages is being prone to the interchanging of samples.

A

Compound Embedding Unit

39
Q

This is for the ease of separation of the tissue block from the mold after it solidifies.

A

Disposable Embeding Molds

40
Q

Tissues are first infiltrated with Celloidin and subsequently embedded in a paraffin mass; For large blocks of dense tissues; obsolete

A

double-embedding method

41
Q

For high resolution light microscopy of thinner than
usual sections, renal biopsies, BM biopsies

A

plastic resin embedding

42
Q

Most widely applied, but carcinogenic due to vinylcyclohexane dioxide (VCD)
component

A

epoxy

43
Q

types of epoxy

A

● Bisphenol A (Araldite) - slow
● Glycerol (Epon) - low viscosity
● Cyclohexene dioxide (Spurr) -
very low viscosity; fastest

44
Q

For electron microscopy; seldom used

A

polyster

45
Q

For high resolution light microscopy

A

acrylic plastics

46
Q

catalyst; forms radicals which are site for polymerization

A

benzoyl peroxide

HISTOPATHOLOGY (16 decks)

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