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Lab techniques 2 > Mycology > Flashcards

Mycology Flashcards

1
Q

Heterotrophs

A

Digest complex organic material (plant/animal) food internally

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2
Q

Heterotrophs may be what?

A

Parasitic or Saprophytic

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3
Q

Parasitic

A

Cause harm to the plant/animal host it is using for food

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4
Q

Saprophytic

A

Feed on dead/dying material

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5
Q

Where may you see saprophytic fungi?

A

Fallen trees

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6
Q

Yeast

A

Single-celled and reproduces by budding

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7
Q

Hyphae

A

Multicellular fungal organisms

Microscopic

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8
Q

Mycelium

A

Large branched webbings

Composed of hyphae

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9
Q

What are the supplies needed for collection & examination of fungal samples

A
  1. Forceps
  2. Swabs
  3. Lactophenol cotton blue
  4. Potassium hydroxide solution
  5. Culture media (DTM and/or Sabouraud agar)
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10
Q

Sabouraud Dextrose

A

Commonly found in most clinics
Does not contain an indicator

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11
Q

What stain confirms identity of fungal organisms?

A

Lactophenol cotton blue

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12
Q

What are the common fungal species?

A

Microsporum canis
Microsporum gypseum
Trichophyton mentagrophytes

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13
Q

Microsporum canis

A

Spores look like canoes and are pointed at the end

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14
Q

Microsporum gypsum

A

Similar to Microsporum canis but is more rounded does nit have the point at the end

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15
Q

Trichophyton mentagrophytes

A

Large and small spores

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16
Q

What percentage of cases with Microsporum canis show evidence of fluorescence?

A

50%

17
Q

How long should a Woods lamp be warmed up for?

A

5 mins

18
Q

What are the 3 ways to test for Dermatophyte testing?

A

Wood’s lamp
Dermatophyte test Medium
Rapid sporulation medium

19
Q

Wood’s lamp

A

Infected hairs will glow in a dark room using fluorescence light

20
Q

Dermatophyte Test Medium (DTM)

A

Culture media will turn in red in the presence of most dermatophytes

21
Q

Rapid Sporulation medium (RSM)/Enhanced sporulation medium (ESM)

A

Culture media will turn teal in the presence of most dermatophytes

22
Q

What color does a positive result for DTM yield?

A

Red

23
Q

If the color change occurs is that 100% positive?

A

No. Confirm with microscopic examination

24
Q

What agar types are used for non-dermatophytes typically?

A

Bismuth-Glucose-Glycine Yeast (Biggy)
Blood agar
Sabouraud dextrose agar

25
Q

How do you do prepare a slide for microscopic evaluation of fungi?

A

Collect mycelium from the media with sticky side of clear cellophane tape

26
Q

What stain do you place on the slide for microscopic evaluation?

A

Lactophenol cotton blue

27
Q

How do you do evaluate fungus microscopically?

A

After placing the lactophenol cotton blue onto the slide, place the tape onto the stain and examine on 10x and 40x

Lab techniques 2 (38 decks)

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