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Lab techniques 2 > Identification Procedures > Flashcards

Identification Procedures Flashcards

1
Q

Motility Tests

A

Wet prep

Hanging drop

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2
Q

How do you prep a wet prep?

A

A moderately heavy suspension of bacteria is made in a few mls of nutrient broth and incubated for 2-3 hours.
Place a loopful of culture on a slide and examine on high dry objective lens

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3
Q

What are you looking for on a wet prep

A

Bacterial motility vs Brownian movement

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4
Q

Brownian movement

A

The random motion of a particle as a result of collisions with surrounding gaseous molecules

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5
Q

Hanging Drop

A

A drop of liquid suspended from a cover glass usually placed over the cavity of a depression slide and containing microorangisms or cells

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6
Q

What is so special about the slides that are being used?

A

They have a concave area that allows the liquid to suspend in order to view motility

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7
Q

What does the motility media allow?

A

The motile bacteria to diffuse out into the medium for detection of motility of bacteria

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8
Q

How do you do a motility test?

A

First tube is incubated at 37 degrees celsius

Second tube is incubated at room temperature

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9
Q

What does non-motile bacteria look like after the tests?

A

Growth is restricted to the stab inoculation line

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10
Q

What does motile bacteria look like after the test?

A

Diffuse growth throughout the medium

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11
Q

When do you use a motile test?

A

If organisms are nonmotile on microscopic examination

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12
Q

Catalase Test

A

Used to determine whether a gram-positive cocci is a staphylococci or streptococci

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13
Q

Positive Catalase Test

A

Bubbles after adding 3% hydrogen peroxide to slide

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14
Q

What bacteria comes up positive on a Catalase Test?

A

Staphylococci

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15
Q

Negative Catalase Test

A

No bubbles

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16
Q

What bacteria come up on a negative catalase test

A

Streptococci

17
Q

Coagulase Test

A

Differentiates coagulase positive Staph and coagulase negative Staph

18
Q

What are coagulase positive bacteria

A

Staphylococcus aureus

Staphylococcus pseudointermedius

19
Q

What are coagulase negative bacteria

A

Staphylococcus epidermidis

Staphylococcus saprophyticus

20
Q

Slide coagulase

A

Detects surface bound coagulase or clumping factor

21
Q

What does a positive tube coagulase look like

A

A clot formation

22
Q

What bacteria comes up positive on a tube coagulase test?

A

Staphylococcus aureus

23
Q

Which enzyme coagulates plasma

A

Coagulase

24
Q

Oxidase Activity

A

The oxidase test identifies organisms that produce the enzyme cytochrome oxidase

25
Q

What bacteria does the Oxidase activity test differentiate

A

Escherichia coli vs Pseudomonas aeruginosa

26
Q

What does a positive oxidase test look like?

A

Microorganisms are oxidase positive when the color changes within 15 to 30 seconds

27
Q

What bacteria does a positive oxidase test indicate?

A

Pseudomonas aeuriginosa

28
Q

What does a negative oxidase activity test indicate?

A

Escherichia coli

No color change

29
Q

For the oxidase activity test, what must be present in the bacteria?

A

Cytochrome Oxidase enzyme

30
Q

Why would you get a false positive oxidase activity test?

A

Because you did not use oxidase reagent that was still clear, or you read your test too quickly once you had performed it

31
Q

Gram positive cocci

Catalase negative

A

Streptococcus spp

32
Q

Staphylococcus epidermidis

A

Gram positive cocci
Catalase positive
Coagulase negative

33
Q

“Golden staph”
Gram positive cocci
Catalase positive
Coagulase positive

A

Staphylococcus aureus

34
Q

Gram negative bacilli

Oxidase positive

A

Pseudomonas aeruginosa

35
Q

Gram negative bacilli

Oxidase negative’

A

Escherichia coli

36
Q

Acid Production for Glucose

A

For fastidious organisms

37
Q

California Mastitis Test

A

Helps confirm the presence of subclinical mastitis

Uses milk, a reagent, and a paddle

38
Q

What are the most common organisms found in the milk?

A 
S. aureus
Streptococcus agalactiae
Streptococcus uberis
E. coli
Corynebacterium spp
Pseudomonas aeruginosa

Lab techniques 2 (38 decks)

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