MT4 - Brucella Flashcards
Rose-Bengal test is used for the detection of antibodies against brucellae
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Slide agglutination test (Rose-Bengal test)
Brucella species cannot tolerate high temperature (60 °C) and low pH (pH: 4).
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Brucellae can be detected by staining in the placenta of aborted foetuses in the case of brucellosis.
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Köster-staining (MZN), Brucella: red, B. ovis & B. canis NO = blue
Humans can acquire brucellosis from raw milk.
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raw milk or dairy products from unpasteurized milk: infected animals.
Europe is free from Brucella ovis
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Undulant fever, malaise, inappetence, fatigue, and muscle and joint pains can be the clinical sings of human brucellosis.
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Brucella species are generally zoonotic agents
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- B. melitensis
- B. abortus
- B.suis
- B.canis
B. ovis = NOT ZOONOTIC
Brucella are facultative IC agents.
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Facultative intracellular pathogens
Brucella is generally a notifiable disease
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Among Brucella species Brucella melitensis is the most pathogenic species for humans.
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- B. melitensis
- B. abortus
- B.suis
- B.canis
More than one serological test must be carried out from one serum sample at the same time in the case of serodiagnostics of brucellosis
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Brucellosis is generally a notifiable disease.
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Brucellosis is a chronic disease:
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Brucella species are gram-negative, fastidious, coccoid rod-shaped bacteria
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There is a serological relationship among some brucella species, because of the same polysaccharides in the cell wall
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Worldwide distribution is characteristic for brucellosis
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Köster staining is used for staining of brucellae
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Brucella can be both acute or chronic in human
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Brucella melitensis is endemic in Mediterranean area
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Endemic :Middle-East, Central Asia, Latin America, Mediterranean Region
Brucella species are widely distributed all over the world.
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Brucella species are serologically uniform
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variations in antigenic structures among different Brucella species.
Brucella species need cysteine or cystine as an additive for the culture
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Brucella species are not zoonotic agents.
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B.ovis: not zoonotic
Susceptibility of humans to different Brucella species is different.
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Consumption of raw milk is an important route of human Brucella infection
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Brucella need chocolate agar to culture.
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There is a close antigenic relationship between B. ovis and B. melitensis.
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B. suis is a zoonotic agent.
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Brucella ovis needs CO2 to culture.
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