Misc Flashcards
Why do you heat fix a slide
Dissolves wax
What are impression smears from
From external lesion a from tissue removed
Disadvantages to impression smears
Few cells collected
Contamination
Only secondary bacterial infection
Types of impressions
Single Ar Tzanck method
Tznack method
-Make multiple impressions of lesion before cleaning
-Clean with sterile saline
-Multiple impressions after cleaning, debridement and scab removal
What to do if there’s a delay in imprinting from collection time
Use scalpel blade used ti expose fresh surface
Where are FNAs collected from
Masses-lymph nodes, modular lesions, organs
FN biopsy site prep
Surgical prep for microbiological testing
FN biopsy from body cavity
Peritoneal, thoracic, joints
Equipment for FNA
- 21-25g needle
- 3-20 lm syringe
Larger needles are used tissue cores tend to
Aspirate
Has greater blood contamination
Things to consider in a tissue biopsy
Location
Accessibility
Nature
+/- clip hair
Site prep
Wedge biopsy obtained with
Scalpel
Wedge biopsy advantages
Large sized specimen
Portion or entire lesion
Punch biopsy sizes
3,4,6,8 mm
4 mm punch
Requires no sutures
6,8 mm punch
Specimen require sutures
Punch biopsy formalin %
10% neutral phosphate buffeted
Endoscopic biopsy collection
Specimen collected by endoscopy flushed from the tip of the endoscope
Endoscopic biopsy sample prep
Blotted on paper towel on am piece of tobgue depressor to create a splint and placed in fixative when dry
Tru-cut biopsy purpose
Biopsy, neoplasia, lesions
Approach of try-cut biopsy
Percutaneous
+/- ultrasound
Aseptic needle instered close to site
Centesis
Introduction of a needle into body cavity to collect fluid
Centesis recording
Total volume collected
Fluid characteristic ( color , turbidity
Transtracheal wash
Sample from trachea, bronchi or bronchioles for do of a pulmonary dz
Process of tracheal wash
Through endotracheal tube through nasal passages, skin, trachea
Why do a transtracheal wash
Minimize pharyngeal contamination of the specimen
Percutaneous technique
18-20 g needle through the catheter
- clip hair of laryngeal area aspectically
- sterilized saline
- when animal coughs pull back plunger to collect fluid
- lidocaine for local anesthesia
- inserted through cricoid membrane asnvabce catheter into tracheal lumen
Orotracheal technique
-Fully under, collected through endotracheal tube
- No cough, withdraw as fluid is injected
Smear from fluid sample prep
Collected in EDTA
- abdominocentesis/thoracocentesis
Starfish smear
Aspirate on slide and smear sample for ideal viscous
