Microscopy

Question 1

What are the qualities of a light microscope?

Answer 1

Poor resolution due to the long wavelength of light.
Living samples can be examined and a colour image is obtained.

Question 2

What are the qualities of a Transmission electron microscope?

Answer 2

High magnification and resolution.
Electrons pass through the specimen to create the image.

Question 3

What are the qualities of a Scanning electron microscope?

Answer 3

High magnification and resolution.
Electrons bounce off the surface of the specimen to create an image.

Question 4

What are the qualities of a Laser scanning confocal microscope?

Answer 4

High resolution and 3D imaging.
Laser light is used to create image.

Question 5

What is resolution?

Answer 5

minimum distance between two objects in which they can still be viewed as separate

Question 6

What is magnification?

Answer 6

refers to how many times larger the image is compared to the object.

Question 7

What are the steps for the process of calibrating a microscope

Answer 7

Step 1- Line up the stage micrometer and eyepiece graticule whilst looking through the eyepiece.
Step 2- Count how many divisions on the eyepiece graticule fit into one division on the micrometer scale.
Step 3- Each division on the micrometer is 10 μm, so this can be used to calculate what one division on the eyepiece graticule is at the current magnification.