Microbiology 4 Flashcards
what is carbons % dry weight, its source and its function?
50%
- organics / CO2
- main constituent of cell, cell material and water
what is oxygens % dry weight, its source and its function?
20%
- H2O, organics, CO2 and O2
- electron acceptor in aerobic respiration
what is nitrogens % dry weight, its source and its function?
14%
- NH3, NO3, organics, N2
- amino acids, nucleotides & coenzymes
what is hydrogens % dry weight, its source and its function?
8%
- H2O, organics, H2
- organic compounds and cell water
what is phosphorous’ % dry weight, its source and its function?
3%
- inorganic phosphates
- nucleic acids, nucleotides & phospholipids
what is sulphurs % dry weight, its source and its function?
1%
- SO4, H2S, S^o and organic sulphur compounds
- proteins & several coenzymes
what is potassiums % dry weight, its source and its function?
1%
- potassium salts
- main inorganic cation & enzymatic cofactor
what is magnesiums % dry weight, its source and its function?
- 5%
- magnesium salts
- inorganic cation & enzymatic cofactor
what is calciums % dry weight, its source and its function?
- 5%
- calcium salts
- inorganic cation, enzymatic cofactors & endospores
what is irons % dry weight, its source and its function?
- 2%
- iron salts
- cytochrome component, enzymatic cofactor
carbon source trophs?
- autotrophs
- heterotrophs
energy source trophs?
- phototrophs
- chemotrophs
electron source trophs?
- lithotrophs
- organotrophs
autotrophs?
CO2 sole / principle carbon source
heterotrophs?
obtained from other organism
phototrophs?
light
chemotrophs?
compound oxidation
lithotrophs?
reduced inorganic compounds
organotrophs?
organic molecules
what are the 2 ways to culture microorganism?
- liquid media (broth)
- solid media (agar plates)
who was in vitro microorganism culture originally grown by?
koch (late C19th) on potato slices and gelatine
in liquid media, how do bacteria grow?
as individual cells until available nutrients exhausted
- makes suspension of cells (can’t differentiate between different cell types without further testing)
on solid media, how does bacteria grow?
bacteria and fungi form colonies with distinctive appearances
- each colony comes from single cells
what can the formula of media influence?
colony appearance (selective and differential media)