Micro Lab Flashcards

1
Q

what must you clean your work station with before you start work and before you leave the lab?

A

2% distell

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2
Q

where must Petri dishes with microbial growth be placed?

A

biohazard bins

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3
Q

why should Petri dish cultures always be marked on the base?

A

tops may become separated from base

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4
Q

why should you write as close to the edge of base as possible?

A

avoid obscuring colonies on growth medium

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5
Q

why must Petri dishes be incubated upside down?

A

prevent condensation falling on cultures and spreading them around dish

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6
Q

what goes in a general waste bin?

A
  • uncontaminated waste
  • hand drying tissue
  • wrappers from sterile items
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7
Q

what goes in a biological waste bin?

A
  • culture plates
  • biological contaminated plastic tube
  • spreaders
  • cuvettes
  • gloves
  • tissues
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8
Q

what goes in a pot with disinfectant?

A
  • biological contaminated plastic pastuer pipettes
  • disposable spreaders
  • disposable loops
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9
Q

what goes in a dispose jar?

A

pipette tips

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10
Q

what goes on the top shelf of a biohazard trolley?

A

items which need autoclaving/decontaminating

e.g. culture, flasks, tubes, universals

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11
Q

what goes on the bottom shelf of a biohazard trolley?

A

storage material

e.g. pipette tip boxes, tube tubs

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12
Q

what does TSA stand for?

A

tryptone soya agar

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13
Q

how big will the zone diameter be (mm) for the organism to be sensitive to antibiotic?

A

more than equal to 20

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14
Q

how big will the zone diameter be (mm) for the organism to have moderate sensitivity to antibiotic?

A

10-19

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15
Q

how big will the zone diameter be (mm) for the organism to be insensitive/resistant to the antibiotic?

A

less than or equal to 9

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16
Q

what must you remember the minimum inhibitory conc is?

A

tube which shows no clear bacterial growth

17
Q

what colour are EMB, mannitol salt and macConkeys agar?

A

red

18
Q

give an example of a bacterial strain that is difficult to culture?

A

mycobacterium tuberculosis