Microbiology 1 Flashcards

1
Q

what is microbiology?

A

the study of microorganisms (microbes)

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2
Q

give examples of how microbiology has a huge impact on a variety of fields?

A
  • fermented foods, baking, brewing, wine making
  • water and sewage treatment
  • agriculture and spoilage
  • study of pathogens and infectious disease
  • pharmaceuticals and biotech
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3
Q

give two characteristics of microorganisms?

A
  • microscopic (<1mm)

- unicellular

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4
Q

are viruses unicellular or acellular?

A

acellular

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5
Q

what type of microorganism can form multicellular structures?

A

fungi

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6
Q

what is the size of a virus?

A

~100nm

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7
Q

what is the size of a bacterium?

A

1-2 micrometres

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8
Q

what is the size of a RBC?

A

~8 micrometres

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9
Q

what is the size of a chicken egg?

A

40-50mm

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10
Q

who has the greatest genetic diversity of all groups of living organisms?

A

microbes

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11
Q

what was the top cause of death between the years 1915 to 2015?

A

infections

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12
Q

when was penicillin G (1st antibiotic) introduced?

A

late 1940s

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13
Q

give examples of how microbes have increasingly shown to have roles in non-infectious diseases?

A
  • stomach ulcers

- obesity

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14
Q

what is the name of the friendly e.coli?

A

Nissle 1917

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15
Q

what is the name of the unfriendly e.coli?

A

O157:H7

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16
Q

what are normal flora?

A

organisms that live in benign symbiosis with host

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17
Q

if conditions change, what may occur?

A

microbes may grow extensively + cause infection - MRSA

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18
Q

what do changed conditions include?

A
  • hormonal
  • climatic
  • enviro
  • stress
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19
Q

how does microbiology play an important role in public health?

A
  • identification of disease
  • tracking and control of disease outbreaks
  • tracking of antimicrobial resistance
  • identifying appropriate treatments
  • vaccination
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20
Q

if resistance is not tackled an estimated …. deaths/year due to AMR infections will occur by 2050

A

10 million

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21
Q

how much economic loss will resistance lead to?

A

60-100 trillion dollars

22
Q

what is biotechnology?

A

the broad area of biology which uses living systems/organisms to produce products

23
Q

colour of agriculture?

24
Q

colour of industrial?

25
colour of food tech?
yellow
26
colour of aquatic?
blue
27
colour of medical and pharmaceutical?
red
28
colour of bioterrorism?
dark
29
what is saccharomyces cerevisiae used for?
bread, beer, wine
30
what is lactobacillus app used for?
yoghurt, bread
31
what is aspergillus niger used in?
citric acid
32
what is bacillus licheniformis used in?
alkaline proteases
33
what is penicillium chrysogenum used in?
penicillin
34
what is streptomycin chrysogenum used in?
streptomycin
35
what is e.coli used in?
insulin (human identical)
36
what is aspergillus niger (fungus) use and what is it used as?
uses raw material: - brewery waste - coconut oil - rapeseed oil used as acidity regulator / flavouring
37
how many tonnes of aspergillus niger are produced?
>1000000 per year
38
what does bacillus licheniformis produce?
alkaline protease in response to nutrient limitation
39
what do alkaline proteases digest?
protein based stains
40
how many tonnes of bacillus licheniformis are produced?
>1000 tonnes per year
41
natural products of antimicrobials?
- penicillins, streptomycin (fungi) | - endolysins (viral)
42
natural product of cholesterol lowering agents?
lovastatin (fungi)
43
natural products of neurotoxins?
botulinum toxin (bacteria)
44
when was insulin (humbling) first produced?
1978 | by genetech then licensed to Eli Lilly
45
what do recombinant vaccines such as Gardasil do?
- target human papilloma virus (HPV) | - contains recombinant viral protein in bacterium
46
what is microbial spoilage?
deterioration of a product by a contaminating microbe
47
how can pharmaceutical products be spoiled by microbes?
- can target raw materials/ finished products - causes economic loss - inc risk to patients by affecting safety and quality
48
what is solid/liquid media?
- nutrient rich | - non-selective
49
how can you use DNA to extract genetic components?
- target regions (PCR) | - whole genome sequencing
50
how can you use RNA to extract genetic components?
gene expression
51
how can you use proteins to extract cell components?
- immunological profiling - structural studies - sequencing