Microbio Chapter 11- The Prokaryotes: Domains of EUbacteria Flashcards
What are the subdivisions of Gram negative bacteria?
- Proteobacteria
- Independent groups (aka Other)
What are the subdivisions of Proteobacteria?
- Alpha
- Beta
- Gamma
- Delta (not important)
- Epsilon
What are the independent groups?
- Chlamydiae
- Bacteroidetes
- Fusobacterium
- Spirochetes
What are the subdivisions of Gram positive bacteria?
Low G+C
High G+C
What is G+C?
Guanine and cytosine ratio
What is the name of LOW G+C bacteria called?
Fermicutes
A Type of Alpha proteobacteria?
Rickettsia rickettsii
Rickettsia rickettsii: Shape
Rods/ Coccobacillus
Rickettsia rickettsii: Respiration
Aerobic
Rickettsia rickettsii: How do they spread?
Insect/ ticks (Are a parasite)
Rickettsia rickettsii: Causes
Spotted fever (Damages Cardiovascular system)
Types of Beta proteobacteria?
A) Bordetella pertussis
B) Neisseria
Bordetella pertussis: Shape
Bacillus
Bordetella pertussis: Respiration
Aerobic
Bordetella pertussis: Structure
Capsule
Bordetella pertussis: Causes
Whooping cough
What are the types of Neisseria? (Beta)
a. Neisseria gonorrhoeae
b. Neisseria meningitis
Neisseria gonorrhoeae: Shape
Dipplococci
Neisseria gonorrhoeae; Respiration
Aerobic
Neisseria gonorrhoeae; Structure
Capsule and fimbrae
Neisseria gonorrhoeae: causes
Gonorrhoea
Neisseria meningitis: Shape
Dipplococci
Neisseria meningitis: Respiration
Aerobic
Neisseria meningitis: Structure
Capsule
Neisseria meningitis: Causes
Meningitis
Types of Gamma proteobacteria?
A) Pseudomonadales
B) Legionellales
C) Vibrionales
D) Enteriobacteriales
E) Pasteurellales
Types of Pseudomonadales?
a. Pseudomonas aeruginosa
b. Moraxella lacunata
Pseudomonas aeruginosa: Shape
Rods
Pseudomonas aeruginosa: Respiration
Aerobic
- The best psychrotrophs
Pseudomonas aeruginosa: Structure
Monotrichous or Lophotrichous flagella
Pseudomonas aeruginosa: Produce pigment?
A blue/green pigment called Pyocyanin is produced by Pseudomonas aeruginosa
Pseudomonas aeruginosa: Causes
- Blue/green Pus in burn patients
- UTI
- Speticema
- Pink eye
Pseudomonas aeruginosa: Spread
Spread easily in hospitals because they love carbon
e.g, Antibiotics and antiseptics
Moraxella lacunata: Shape
Coccobacilli
Moraxella lacunata: Respiration
Aerobic
Moraxella lacunata: Causes
Pink eye
Types of Legionellales?
a. Legionella pneumophila
b. Coxiella burnetti
Legionella pneumophila: Shape
Bacillus/ Rods
Legionella pneumophila: Respiration
Aerobic
Legionella pneumophila: Spread by?
Contaminated water sources
e.g; AC units, Hot water tanks, shower heads
-not spread via humans
Legionella pneumophila: Causes
2 forms of Pneumonia:
1. Legionaire’s disease (Fatal)
2. Pontiac fever (mild)
Coxiella burnetti: Shape
Bacillus/ rods
Coxiella burnetti: Respiration
Aerobic
Coxiella burnetti: Structure
ENDOSPORES
-Only gram-neg bacteria with endospores
Coxiella burnetti: Spread by?
Harbored in Animals (Cattle) and spread through unpasteurised milk and aerosols
Coxiella burnetti: Causes
Q-fever (type of pneumonia)
A type of Vibrionales?
Vibrio cholerae
Vibrio cholerae: shape
Vibrio (curved rod)
Vibrio cholerae: respiration
Facultative anaerobic
-Grow faster when O2 is present but can still grow without O2
Vibrio cholerae: Structure
Monotrichous flagella
Vibrio cholerae: Causes
Rice-water stools
Types of Enteriobacteriales?
a. Escherichia coli
b. Salmonella
c. Shigella dysenteriae
All Enteriobacteriales; Shape
Bacillus (rods)
All Enteriobacteriales: Respiration
Facultative anaerobes
All Enteriobacteriales: Structure
Peritrichous flagella
Pili/pilus
What is an enteric?
All Enteriobacteriales
-GI tract loving
Kill and destroy all other bacteria
All Enteriobacteriales: produce
Bacteriocin
-Peptidic toxins produced by bacteria to inhibit the growth of a closely related strain
why is Escherichia coli a lab pet?
Grows easily
What do regulatory agencies test for in water?
Coliform
Escherichia coli: Causes
Travellers diarrhoea (hospitals)
UTI
HUS (Haemolytic uremic syndrome)
What does HUS cause?
hemolysis and kidney failure
Escherichia coli: Spread by?
Food borne illness
e.g; ground beef
What are the types of Salmonella?
a. Salmonella typhi
b. Salmonella enterica
Salmonella enterica: Spread by?
Food source
Salmonella typhi: Spread by?
A human carrier
Salmonella typhi: Causes
Severe GI infection
What does Shigella dysenteriea produce?
Shiga toxin
What is shiga toxin?
A bacterial protein toxin that inhibits protein synthesis that destroys cell which leaves a scar, most commonly in the intestines
Shigella dysenteriea: Spread by?
Fecal matter
-commonly spread in day cares
What is a type of pasteurellales?
Hemophilus influenza
Hemophilus influenza: Shape
Coccobacilli
Hemophilus influenza: Respiration
Aerobic
Haemophilus influenza: Structure
Capsule
Why is Haemophilus influenza blood-loving?
The bacteria uses the X factor and V factor from the blood to grow. As the bacteria itself is missing both factors.
What is the X factor?
Cytochrome
What is the V factor?
NAD or NADP
Haemophilus influenza: Causes
Meningitis
Otitis media
Pneumonia
—> NOT INFLUENZA!! <—
What are types of epsilon proteobacteria?
- Helicobacter pylori
- Complyobacter jejuni
Helicobacter pylori: Shape
Vibrio
Helicobacter pylori: Respiration
Microaerophile
Helicobacter pylori: Structure
Peritrichous flagella
Helicobacter pylori: Causes
Stomach ulcers
Complyobacter jejuni: Shape
Vibrio
Complyobacter jejuni: respiration
Microaerophile
Complyobacter jejuni: Structure
Monotrichous flagella
Complyobacter jejuni: Causes
Gastroenteritis
What is a common type of Chlamydiae?
Chlamydiae trachomatis
Chlamydiae trachomatis: Shape
Coccobacilli
Chlamydiae trachomatis: respiration
Aerobic
Chlamydiae trachomatis: Spread by?
Airborne routes or interpersonal contact
e.g Fomites
Chlamydiae trachomatis: causes
Trachoma (Blindness)
Non-gonococcal urethritis (NGU)
Lympho-Granuloma Venereum
How long is the Chlamydiae life cycle?
48 hours
What does the Chlamydiae start off as in their life cycle?
An elementary body
What is an elementary body?
A bacteria’s infectious form that attaches to a host cell
What does the elementary turn into after the host cell phagocytizes it? (Chlamydiae life cycle)
The elementary body is housed inside the vacuole and it then reorganises into a RETICULATE BODY
What do the reticulate bodies do during the Chlamydiae life cycle?
They multiply and then convert back to elementary bodies which are then released from the host cell and the cycle continues
Bacteroidetes species: Shape
Rods/ bacillus
Bacteroidetes species: Respiration
Anaerobic
Bacteroidetes species: Causes
Gingivitis and Peritonitis
What is Gingivitis?
Pus forms between the tooth and gum causing inflammation and eventually could result in losing teeth
Fusobacterium species: Shape
Bacillus/rods
Fusobacterium species: Respiration
Anaerobes
Fusobacterium species: Causes
Gingivitis
What is a type of Spirochetes?
Treponema pallidum
Treponema pallidum: Causes
An STD (Syphilis)
How is Treponema pallidum grown?
Only in live bodies e.g; Rabbits
(No in vitro)
Why could there be a potential vaccine for Treponema pallidum?
Because it’s genome is fully mapped
What are the types of Firmicutes? (Low G+C gram positive)
- Clostridiales
- Bacillales
- Lactobacillales
4.Mycoplasmatales
What are the types of Clostridiales?
A. Clostridium
B. Clostridioides
What are specific types of Clostridium?
Clostridium tetani
Clostridium botulinum
Clostridium perfringens
What is a specific type of Clostridioide?
Clostridioides difficile
All clostridiales: Shape
Bacillus (rods)
All clostridiales: Respiration
Anaerobes
All clostridiales: Structure
Endospore
“Lollipop rods”
All clostridiales: Spread by?
Soil
What are specific types of Bacillales?
A. Bacillus anthracis
B. Staphylococcus aureus
Bacillus anthracis: Shape
Bacillus (rods)
Bacillus anthracis: Respiration
Facultative anaerobe
-Still considered aerobic
Bacillus anthracis: Structure
Endospores
Bacillus anthracis: Spread by?
Soil
What was Bacillus anthracis used as during 9/11?
A bioweapon
Bacillus anthracis: Causes
Anthrax
Staphylococcus aureus: Shape
Clusters (Staphylococcus)
Staphylococcus aureus: Respiration
Facultative anaerobe
Staphylococcus aureus: Grows in?
Special media
Staphylococcus aureus: Colour
Gold
Staphylococcus aureus: Causes
Skin infections (Sty), Toxic shock syndrome and food poisoning
Types of Lactobacillales?
A. Lactobacillus
B. Streptococcus
C. Listeria
D. Enterococcus
Are Lactobacillus sp. pathogenic?
Not really
Lactobacillus sp.: Respiration
Aerotolerant anaerobes (Fermenters)
Lactobacillus sp.: Shape
Bacillus
Where is Lactobacillus sp. used?
Commercially and in hospitals
What is Lactobacillus sp. commercial use?
Probiotics
What is Lactobacillus sp. hospital use?
For women health
What does Lactobacillus sp. do in regards to women’s health?
-During puberty estrogen triggers the production of Lactobacilli in the vagina to create a sterile environment (acidic)
-During pregnancy, lactobacilli is used as a protective mechanism (Increases population) as it the first bacteria a newborn comes in contact with
All Streptococcus are in chains EXCEPT?
Streptococcus pneumoniae
Streptococcus pneumoniae: Shape
Dipplococci
Streptococcus pneumoniae: Structure
Capsule
Types of streptococcus?
A. Streptococcus pyogenes
B. Streptococcus mutans
C. Streptococcus pneumoniae
Streptococcus pneumoniae: Causes
Pneumonia
Meningitis
Otitis media
Streptococcus pyogenes: Shape
Streptococcus (a Chain)
all Streptococcus: Respiration
Aerotolerant
Streptococcus pyogenes: Structure
Doesn’t have a capsule to attach, instead uses M-protein
Streptococcus pyogenes: Causes
Skin infections
Strep throat
Scarlet fever
Rheumatic fever
Otitis media
Streptococcus pyogenes: Grows in?
Blood agar
-Produces clear zones due to beta- hemolysis
Streptococcus mutans: Shape
Streptococcus (Chains)
Streptococcus mutans: structure
Capsule
Streptococcus mutans: Causes
Tooth decay
Streptococcus mutans and Streptococcus pneumoniae: Grow in?
Blood agar
-Non-beta haemolytic
What is a type of listeria?
Listeria monocytogenes
Listeria monocytogenes: Shape
Bacillus
Listeria monocytogenes: Respiration
Facultative anaerobe
Listeria monocytogenes: grow in what temperature?
Cold
-Psychotrophs
Listeria monocytogenes: Spread by
Spoiled foods
-Cold cuts (deli meat)
-Premade Salad
-Soft cheeses
Listeria monocytogenes: Causes
Listeriosis (food borne illness, food poisoning like symptoms)
Why is Listeria monocytogenes dangerous during pregnancy?
If infected the mother may have a miscarriage, still born or an ill newborn
What are some types of Enterococcus bacteria?
A. Enterococcus faecalis
B. Enterococcus faecium
Enterococcus: Shape
Coccus
Enterococcus: Respiration
Facultative anaerobe
Why are enterococcus described as hardy?
They are very resistant and can survive easily in air, bedding, hands
-Healthcare-associated infections
Enterococcus: Causes
Catheter infections
UTI
Surgical wound infections
Enterococcus: spread by?
Fecal matter
Type of mycoplasma?
Mycoplasma pneumoniae
Why are mycoplasma atypical?
Have NO cell wall
-Pleomorphic
Mycoplasma pneumoniae: Appearance
Fried-egg shape
Mycoplasma pneumoniae have _______ in their plasma membrane
Sterols
Mycoplasma pneumoniae: Causes
Walking pneumonia
-milder lung infection
-Young adults are more susceptible
How is walking pneumonia treated?
Tetracycline
What are types of HIGH G+C bacteria?
- Mycobacterium sp.
- Corynebacterium
- Propionibacterium sp.
Mycobacterium contain?
Mycolic acid
Mycobacterium: Shape
Bacillus (rods)
Mycobacterium: respiration
Aerobic
Mycobacterium use what kind of stain?
Acid-fast stain
-Their diagnosis is more reliable
What are Mycobacterium resistant to because of the mycolic acid?
Desiccation
Antiseptics
Antibiotics
How do Mycobacterium grow?
Grow very slowly: Nutrients enter slowly making colonies appear at around 20 hours of growth
Examples of Mycobacterium?
Mycobacterium leprae (Leprosy)
Mycobacterium tuberculosis
Corynebacterium diphtheriae: Shape
Bacillus (rods)
Corynebacterium diphtheriae: Respiration
Facultative anaerobe
Corynebacterium diphtheriae: Staining appearance
Picket fence or Chinese alphabet because they are pleomorphic
Corynebacterium diphtheriae: Site of colonisation appearance
Pseudomembrane forms causing a leather like appearance
Corynebacterium diphtheriae: Causes
Diphtheria
(An upper respiratory tract infection)
Is Corynebacterium diphtheriae preventable?
Yes, the D Tap vaccine provides immunity against infection
What is a type of Propionibacterium?
Propionibacterium acnes
Propionibacterium acnes: Shape
Bacillus
Propionibacterium acnes: Respiration
Anaerobic
Why is Propionibacterium used for Swiss cheese?
They can be fermenters
How does Propionibacterium affect Swiss cheese?
Adds flavour (nutty) and creates holes (CO2)
What is the end product that creates Swiss cheese flavour?
Propionic acid
Propionibacterium acnes: Causes
Acne
What else is involved in acne formation other than bacteria?
Hormones
How is Propionibacterium acnes treated?
Can be treated without use of antibiotics
e.g; Benzoyl peroxide, routine cleaning