Microbial Growth Deck (Practical)

Question 1

Name the 5 I’s employed by microbiologists.

Answer 1

1) Inoculation
2) Isolation
3) Incubation
4) Inspection
5) Identification

Question 2

Placing a specimen onto an artificial culture medium

Answer 2

What is inoculation?

Question 3

Spatially separating microbial growth form distinct CFUs

Answer 3

What is isolation?

Question 4

Placing the culture medium in a particular environment to promote growth

Answer 4

What is incubation?

Question 5

Examining culture for the presence of microbial growth

Answer 5

What is inspection?

Question 6

Performing specific tests to ID any pathogenic microbes isolated

Answer 6

What is identification?

Question 7

Media containing nutrients in unknown quantities that are added to bring about a particular characteristic of a microbial strain

Answer 7

What is complex media?

Question 8

General purpose media that supports the growth of most non-fastidious organisms

Answer 8

What is non-selective media?

Question 9

Media that enhances the growth of certain (fastidious) bacteria and inhibits the growth of others

Answer 9

What is selective media?

Question 10

Media that allows dissimilar bacteria to be distinguished from each other based on physical appearance or colony morphology

Answer 10

What is differential media?

Question 11

Growth medium suitable for the culture of microbes or animal cells (including human) of which all of the chemical components are known

Answer 11

What is chemically-defined media?