Bacterial Culture & Growth Deck (Exam 1)

Question 1

What are the 3 main requirements of all microbes?

Answer 1

1) Macroelements
2) Microelements
3) Growth factors

Question 2

This type of microbe thrives in extreme heat.

Answer 2

What is a hyperthermophile?

Question 3

This type of microbe thrives in heat in general.

Answer 3

What is a thermophile?

Question 4

This type of microbe thrives in really cold environments.

Answer 4

What is a psychrophile?

Question 5

This type of microbe thrives in very moderate temperatures.

Answer 5

What is a mesophile?

Question 6

This type of microbe loves a low-pH environment.

Answer 6

What is an acidophile?

Question 7

This type of microbe loves a high-pH environment.

Answer 7

What is an alkaliphile?

Question 8

This type of microbe loves high-salt environments.

Answer 8

What is a halophile?

Question 9

The { } temperature is one in which bacteria have the { } generation time i.e. grow the best.

Answer 9

1) Optimal
2) Shortest

Question 10

The { } of a bacterial growth curve represents the optimal temperature.

Answer 10

Peak

Question 11

The most common food spoilage organisms are…

Answer 11

Mesophiles

Question 12

Any substance used to grow bacteria e.g. broth or agar.

Answer 12

What is a culture medium?

Question 13

This type of microbe thrives in high-pressure environments.

Answer 13

What is a barophile?

Question 14

What is the optimal pH range?

Answer 14

6.5 to 7.5

Question 15

This is placed in/on the medium to start growth.

Answer 15

What is the inoculum?

Question 16

Bacterial growth in/on the medium.

Answer 16

What is a culture?

Question 17

The measure of relative clarity of a liquid/broth.

Answer 17

What is turbidity?

Question 18

This type of media supports the growth of most bacteria that are not fastidious i.e. picky.

Answer 18

What is non-selective?

Question 19

This type of media encourages the growth of obligate anaerobes.

Answer 19

What is reduced?

Question 20

This type of media inhibits some bacteria and encourages others.

Answer 20

What is selective?

Question 21

This type of media can distinguish between bacteria based on colony type.

Answer 21

What is differential?

Question 22

This type of media promotes the growth of pathogens in low numbers and inhibits others present.

Answer 22

What is enrichment?

Question 23

This type of media promotes the growth of most chemoheterotrophic organisms.

Answer 23

What is complex?

Question 24

Microbial growth refers to the increase in the { } of cells.

Answer 24

Number

Question 25

Generation time refers to the { } of the bacterial population over time.

Answer 25

Doubling

Question 26

What are the 4 phases of a bacterial growth curve?

Answer 26

1) Lag
2) Logarithmic
3) Stationary
4) Death/logarithmic decline

Question 27

A closed system will exhibit the { } growth curve while the continuous/open system remains in the { } phase.

Answer 27

1) Standard
2) Logarithmic

Question 28

List the types of aerobic/anaerobic growth:

Answer 28

1) Obligate aerobes
2) Facultative anaerobes
3) Obligate anaerobes
4) Aerotolerant anaerobes
5) Microaerophiles

Question 29

What is the difference between facultative anaerobes and aerotolerant anaerobes?

Answer 29

Facultative anaerobes - grow w/ or w/o oxygen but grows better w/ oxygen

Aerotolerant anaerobes - grow only w/ oxygen but will continue in presence of it