Evaluation of Disinfectants & Antiseptics Deck (Practical)

Question 1

What is the difference between antiseptics and disinfectants?

Answer 1

Antiseptics can be used on living tissue while disinfectants are used on inanimate objects

Question 2

What is the difference between a sterilizing agent and a sanitizing agent?

Answer 2

Sterilizing agents destroy all microbial lifeforms while sanitizing agents reduce the number of microbes to an acceptable amount

Question 3

What are some physical/non-chemical methods of controlling microbial growth? Name 3.

Answer 3

1) Temperature
2) pH
3) UV light

Question 4

Method in which a piece of filter disk paper with some antimicrobial agent is placed on an inoculated agar plate to test the effectiveness of said agent.

Answer 4

What is the disk diffusion method?

Question 5

An area of media where bacteria are unable to grow, due to presence of a drug or antimicrobial agent that impedes their growth

Answer 5

What is the zone of inhibition?

Question 6

What is the relationship between the size of the zone of inhibition and the effectiveness of an antimicrobial agent?

Answer 6

^ size of zone of inhibition = ^ effectiveness of antimicrobial agent

Question 7

When performing the disk diffusion method, it is important that the agar media is { } covered and that the plate is { } several times for adequate coverage.

Answer 7

1) Uniformly
2) Rotated