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Microbiology > Micro Lab Practicle > Flashcards

Micro Lab Practicle Flashcards

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Biology 101 flashcards
0
Q

What is room temp for incubation?

A

25C

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1
Q

What is body temp for incubation?

A

37C

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2
Q

Why is the position of the plate important?

A

To keep condensation from dripping and causing the bacteria to run together

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3
Q

What materials are used in a grams stain?

A

Crystal violet, grams iodine, decolorizing alcohol, safranin red

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4
Q

What does a grams stain tell us?

A

Morphology, positive or negative, arrangement

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5
Q

Which stain do you not heat fix?

A

Negative stain

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6
Q

What can a negative stain tell us?

A

Morphology only

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7
Q

Whatdoes a spore stain do?

A

Helps determine if bacteria are spore formers

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8
Q

What materials are used in a endosperm stain?

A

Malachite green, alcohol, and safranin red

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9
Q

What is used in a negative stain?

A

India ink

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10
Q

What is a capsule stain used for?

A

To determine if there is a capsule

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11
Q

What do you use in a. Capsule stain?

A

India ink

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12
Q

What is the trickiest but most important part of a gram stain?

A

Decolorizing alcohol

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13
Q

What does counter stain mean?

A

Used to help differentiate different types of bacteria

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14
Q

What is a mordant?

A

Locking mechanism

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15
Q

What does gram variable mean?

A

Not sure if it is positive or negative, will be not quite purple or pink

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16
Q

What is an acid fast stain good for?

A

Good for bacteria that have a lipid cell wall that doesn’t stain

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17
Q

What is a bacterial capsule?

A

A gelatinous material coating the outside of the cell. A thick glycocalyx

18
Q

What is differential media?

A

Helps differentiate between bacteria, pos or neg, and what they favor

19
Q

What is selective media?

A

Prohibits or inhibits certain types of growth

20
Q

What is alpha growth?

A

Kinda used blood

21
Q

What is beta growth?

A

Used all blood

22
Q

What is gamma growth?

A

No blood

23
Q

What is osmolarity?

A

Bacterial growth in different concentrations of saline

24
Q

What is transduction?

A

Process in which phage infects bacteria

25
Q

What is meant by a lawn?

A

Evenly and adequately covering an area with bacteria

26
Q

What is a mueller Hinton agar?

A

Helps determine bacteriostatic or bacterialcidal

27
Q

What units to you use to measure the zones?

A

Mm

28
Q

Why would you preform a serial dilution exercise?

A

To lower microbe count to a countable level

29
Q

What does strepto mean?

A

Chains

30
Q

What does staphylo mean?

A

Bunches

31
Q

What does palisade mean?

A

Rods

32
Q

What does diplo mean?

A

Pairs

33
Q

What is the mueller Hinton agar?

A

Antibiotic sucrptibility

34
Q

What would you use a broth agar for?

A

Large number of bacteria

35
Q

What would you use a plate agar for?

A

Big surface area

36
Q

What would you use a slant agar for?

A

Smaller storage and it does not dry it as easy

37
Q

What would you use an agar deep for?

A

Decide if it is aerobic, anaerobic, or facultative

38
Q

What is a microorganism that an acid stain is good against?

A

Mycobacterium TB

39
Q

What is a vegetative cell?

A

An active cell

40
Q

What is a endo spore?

A

Inactive spore

41
Q

What color will a vegetative cell be?

A

Red

42
Q

What color will a endo spore be?

A

Green

Microbiology (22 decks)

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