Micro: Gram Stain

Question 1

Why are stained smears used?

Answer 1

Lots of information cheaply, quickly

Question 2

When are gram stains used?

alone or with other tests

Answer 2

Can be used in conjunction with other tests or alone

Question 3

What is important to do when doing a gram stain? (2)

Answer 3

1. clean slide

2. heat fix

Question 4

What are the gram stain components? (stains) (4)

Answer 4

1. Crystal Violet - 30 sec
2. Gram’s Stain - 30 sec
3. Decolorize - 10 sec
4. Counterstain - 30 sec

Question 5

Is this Gram + or -?

1. Blue/purple
2. Retains 1st stain

Answer 5

Gram +

Question 6

Is this Gram + or -?

1. Red
2. Decolorized
3. Picks up counterstain

Answer 6

Gram -