Micro: Culture Media

Question 1

What type of media is this?

1. Agar or broth
2. Supports the growth of many pathogens
3. Basic media, not selective
4. Nutrient agar/broth, trypticase soy

Answer 1

Nutrient Media

Question 2

What type of media is this?

1. Inhibit all but a few types of bacteria
2. Facilitate isolation of particular bacteria

Answer 2

Selective Media

Question 3

What agar is this?

Isolation of salmonellosis

Answer 3

Brilliant Green Agar

Question 4

What agar is this?

1. Suppresses G(+)
2. Grows G(-)

Answer 4

MacConkey Agar

Question 5

What agar is this?

Not used routinely but highly selective for staphylococcus

Answer 5

Mannitol Salt Agar

Question 6

What type of media is this?

1. Can be built into selective media
2. Indicator changes visibly with metabolic activity of bacteria

Answer 6

Indicator Media

Question 7

What agar is this?

1. Supports growth of most pathogens
2. Hemolytic activity for pathogens

Answer 7

Blood Agar

Question 8

What type of hemolytic activity is this?

1. Utilizing RBC for energy
2. Will have a clear zone around growth

Answer 8

Beta

Question 9

What type of hemolytic activity is this?

1. Partially utilize RBC for energy
2. Greenish hazy appearance around growth
3. Green = hemoglobin breakdown, biliverdin

Answer 9

Alpha

Question 10

What type of hemolytic activity is this?

1. No hemolysis
2. No blood used

Answer 10

Gamma

Question 11

What type of indicator media agar is this?

1. Lactose, pH indicator
2. Isolates acid producing bacteria E. coli, Klebsiella
3. Plus bile salts
4. Selective for enterobacteria
5. G(-) rods

Answer 11

McConkey

Question 12

What type of agar is this?

1. Kirby-Bauer technique
2. Antimicrobial susceptibility testing with antibiotic discs

Answer 12

Mueller-Hinton Agar