MCB Lecture 22 Gene Expression II Flashcards

0
Q

Describe the function of haemoglobin

A

It carries four oxygen molecules per haemoglobin. It transports oxygen in the blood from lunch to tissue

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1
Q

Describe the structure of haemoglobin

A

Two alpha subunits, two beta subunits

It’s a tetramer

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2
Q

Describe the evolution of globin genes

A

There was a single gene
500 years ago: alpha and beta diverged
300 years ago: beta became epsilon, gamma, deta and beta

There are pseudogenes: chi globin

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3
Q

Describe the features of the beta globin gene cluster

A

It is highly conserved
Has pseudogenes
Has LINEs and SINEs

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4
Q

What are the chi genes an example of?

A

Pseudogenes

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5
Q

What is the epsilon gene?

A

It is the embryonic version of beta globin

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6
Q

What is the delta gene?

A

It is an adult beta globin, it has very low expression

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7
Q

What is the gamma gene?

A

It is the foetal globin instead of beta.

It has a high affinity for oxygen

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8
Q

What is the beta gene?

A

It is the adult version of the globin

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9
Q

How many copies of the alpha gene are there?

A

2

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10
Q

Describe the differential expression of the different globin genes during development. This is a classic example of …

A

Statiotemporalexpression

Embryo yolk sac: epsilon
Foetus, liver: gamma
Bone. Murrow in adult: beta

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11
Q

What controls the statiotemporalexpression of the globin genes?
Describe how this mechanism works

A

LCR
This is an enhancer that selectively activates either epsilon, gamma or beta globin

  1. Tissue specific signals bind to LCR
  2. Pre-initiation complex forms
  3. Appropriate gene is transcribed
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12
Q

What is an insulator?

A

It makes sure that the enhancer doesn’t activate inappropriately certain genes

The two insulators come together, forming a loop where all the globin genes are.

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13
Q

What are haemoglobinopathies?

Give examples

A

These are disorders of haemoglobin

Sickle cell anaemia
Alpha and beta thalassaemia

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14
Q

Describe the features of sickle cell anaemia

A

The cell is sickled and blocks arteries and can’t deliver enough oxygen

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15
Q

Describe how SCD arises. What is the mutation?

A

There is a mutation in the beta globin gene.

When the haemoglobin is synthesised, they all stick together in a rigid chain

16
Q

Describe the mutation and how it arises in alpha thalassaemia

A

There is unequal crossing over of the alpha thalassaemia genes and then there is a reduced number of alpha globin genes

17
Q

Describe the 5 categories of alpha thalassaemia

A
Four copies: Normal 
3 copies: carrier
2 copies: minor
1 copy: major
0 copies: death
18
Q

Describe the possible mutations leading to beta thalassaemia

A

SNP in promoter region, 5’ UTR, intron consensus sequences

19
Q

What is important about beta thalassaemia and new born babies?

A

Newborn babies appear normal because they aren’t using beta globin yet, (still gamma)

The beta thalassaemia occurs later

20
Q

Compare oxygen affinity in gamma and beta globin

A

Gamma has a higher affinity for oxygen