MCB Lecture 2 Building Blocks Flashcards

0
Q

What are some general functions of protein?

A
Structure
Enzymes
Hormones (signalling molecules)
Membrane receptors
Channels
Molecular machines
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1
Q

What proportion of the dry weight of cells does protein make up?

A

Half of the dry weight of a cell

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2
Q

What is GFP and what is its significance?

A

Green fluorescent protein
Illustrates the variety of properties that proteins have.
This protein fluoresces all by itself : no chromophore bound
E amino acid sequence produces colour, and guides protein folding

Single point mutations give other colours

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3
Q

What is molarity?

A

Concentration of solutes in solvents

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4
Q

What are the weak non covalent bonds?

A

Hydrogen bonds
Van Der Waals interactions
Hydrophobic force

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5
Q

What is free energy?

A

Energy available in a cell that is accessible for chemical reactions

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6
Q

What is delta G?

A

Change in Gibbs’ free energy

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7
Q

What is an exogenous reaction, and what is delta-G for exogenous reactions?

A

Release of energy

Negative delta G

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8
Q

What is an endergonic reaction, and what is delta-G for endergonic reactions?

A

Absorption of energy

Positive delta G

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9
Q

How is delta-G related to delta-H and delta-S?

A

Delta-G = delta-H - T x delta-S

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10
Q

In terms of entropy and heat, what are the requirements for an exogonic reaction?

A

Big release of heat or big increase in entropy

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11
Q

What does positive delta-H represent?

A

Absorption of energy

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12
Q

What does negative delta-H represent?

A

Release of heat

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13
Q

What does negative delta-S represent?

A

Decrease in entropy

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14
Q

What does positive delta-S represent?

A

Increase in entropy

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15
Q

Why is delta-G (change in Gibbs’ free energy) a useful thing for biochemistry?

A
  1. Predict spontaneous reactions
  2. Position of equilibrium
  3. Tightness of binding
16
Q

What is delta-G nought primed?

A

This is the stand free energy change for a reaction

17
Q

How does delta-G nought primed relate to K (equilibrium constant)?

A

Delta-G nought primed = - R T lnKd

18
Q

What is the relationship between equilibrium and dissociation constants? (K and Kd)

A

They are reciprocals

19
Q

What does [L] = Kd mean?

A

Half of the substrate is bound to the enzyme

20
Q

What is the difference between delta-G and delta-G nought primed?

A

Stand lab conditions vs. standard biochemical conditions

21
Q

What are the conditions for delta-G nought primed?

A

pH 7

[h2o] = 55.5 M

22
Q

What are some of the ways that unfavourable reactions can be made to go?

A
  1. Couple with highly favourable reaction
  2. Remove product
  3. Have high concentration of reactant
23
Q

How does Kd relate to tightness of binding?

What indicates weak, intermediate and strong binding?

A

Smaller the Kd, the tighter the substrate-enzyme binding
Weak: mM
Intermediate: microM
Tight: nM