MANUAL CELL COUNT

Question 1

Manual cell count uses hemocytometer, most common is the ________

Answer 1

Levy chamber with improved Neubauer ruling

Question 2

Each grid has nine 1 mm x 1 mm squares, with a total area of ___ mm2

Answer 2

9

Question 3

Each of the four corner Large Squares is subdivided into

Answer 3

6 small squares

Question 4

Center Large Square is subdivided into

Answer 4

25 small squares

Question 5

Distance between each counting surface and the coverslip is

Answer 5

0.1mm

Question 6

Formula of Manual Cell Count:

Answer 6

Cells counted x Dilution factor / Area counted x 0.1mm

Question 7

WBC Counting- 4 large corner squares
Area of 1 WBC square

Answer 7

1 mm2

Question 8

RBC Counting- 5 smalls squares from the central large square
Area of 1 RBC square

Answer 8

0.04mm2

Question 9

Platelet counting- whole area of central large square
Area of 1 central large square

Answer 9

1 mm2

Question 10

Blood is aspirated until ______ unit depending on the dilution in RBC pipette

Answer 10

0.5 or 1.0

Question 11

Diluent is aspirated until the ___ unit RBC pipette

Answer 11

101

Question 12

Diluent is aspirated until the ____ unit in WBC pipette

Answer 12

11

Question 13

RBC Pipette
units:
dilutions:

Answer 13

0.5, 1.0, 101
1:100, 1:200

Question 14

WBC Pipette
units:
dilutions:

Answer 14

0.5, 1.0, 11
1:10, 1:20

Question 15

WBC count
Diluting Fluids:

Answer 15

1% ammonium oxalate
3% acetic acid
1% HCl

Question 16

RBC count
Diluting Fluids:

Answer 16

NSS

Question 17

Platelet count
Diluting Fluids:

Answer 17

1% ammonium oxalate

Question 18

RBC count
Dilution:

Answer 18

1:100

Question 19

WBC count
Dilution:

Answer 19

1:20
1:100

Question 20

Platelet count
Dilution:

Answer 20

1:100

Question 21

WBC count
Objective lens:

Answer 21

10x or LPO
10x

Question 22

RBC count
Objective lens:

Answer 22

40x or HPO

Question 23

Platelet count
Objective lens:

Answer 23

40x or HPO

Question 24

WBC count
Area counted:

Answer 24

4mm2

Question 25

RBC count
Area counted:

Answer 25

0.2mm2

Question 26

Platelet count
Area counted:

Answer 26

1mm2

Question 27

T or F
Acid in the WBC diluting fluid is used to lyse the RBC

Answer 27

T

Question 28

PROCEDURE FOR WBC COUNT:
Allow dilution to sit for _____ (complete lysis of RBCs)

Answer 28

10 MINUTES

Question 29

PROCEDURE FOR WBC COUNT:
Place hemacytometer in a moist chamber for _______ (allows cells to settle)

Answer 29

10 MINUTES

Question 30

If _______ NRBCs per 100 WBCs are observed on the differential count on a stained peripheral blood film, the WBC count must be corrected.

Answer 30

five or more

Question 31

Formula for Corrected WBC Count:

Answer 31

Uncorrected WBC count x 100 / #nRBCs + 100