PERIPHERAL BLOOD FILMS Flashcards
PERIPHERAL BLOOD FILMS:
Should be made within _____ after blood collected using EDTA.9/L
3-4 hours
Possible problems that can be encountered in certain conditions:
EDTA-induced platelet ____
EDTA-induced platelet ____
satellitosis
clumping
Most convenient and most used technique type
Wedge method
Requires at least two glass slides
Wedge method
Wedge method
dimensions:
75 x 25 mm or 3-inch x 1 inch
Wedge method
Size of drop of blood:
2-3 mm
Wedge method:
Distance of blood from edge of slide:
1 cm
Wedge method:
Angle of pusher slide:
30-45 degrees
< 30 degrees angle of smear
Thinner
> 30 degrees angle of smear
Thicker
Wedge method
Length:
2/3 or 3/4 of slide
Wedge method
Drying of smear:
Small electric fan
Widely used stains:
Polychromes stains
basic dye stains acidic cellular component (e.g.
nucleic acids)
Methylene blue
acidic dye and stains basic cellular components (e.g. hemoglobin)
Eosin
Fixative:
Methanol
aka Romanowsky stain
Wright’s stain
Methyl alcoholic solution of:
Eosin
Methylene blue (50-75%)
Azure B
Buffer solution (K+ phosphate / Na phosphate, pH ____)
6.4
Too alkaline buffer
excessive blue stain
Prolonged staining time
excessive blue stain
Inadequate washing
excessive blue stain
Thick films
excessive blue stain
Too acidic buffer
excessive pink stain
Insufficient staining time
excessive pink stain
Prolong washing
excessive pink stain
Mounting coverslips before they are dry
excessive pink stain
HPO
WBC estimation can be performed (Factor used:____)
2000
HPO
Ave. of 5 WBCs/HPF x 2000 =
10,000/uL
OIO
“_______” track pattern is preferred to minimize distribution errors
Battlement
Platelet estimation can be performed (Factor used: _____)
20,000
T or F
Males have less RBC and Hct than females due to hormones (testosterone)
F
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