HEMOGLOBIN DETERMINATION

Question 1

20 uL blood + 0.1N HCI =

Answer 1

Acid Hematin (Sahli-Hellige method)

Question 2

T or F
Hb F, Hi, HbCO and SHb can be converted into acid hematin

Answer 2

F
cannot

Question 3

Procedure: 20 uL of blood + 0.1N NaOH =

Answer 3

Alkali Hematin

Question 4

______ resistant to alkali denaturation

Answer 4

Hb Fis

Question 5

Visual Colorimetry for HEMOGLOBIN DETERMINATION

Answer 5

Acid Hematin (Sahli-Hellige method)
Alkali Hematin

Question 6

Photoelectric Colorimetry for HEMOGLOBIN DETERMINATION

Answer 6

Cyanmethemoglobin method (HICN)

Question 7

AKA Hemoglobin cyanide

Answer 7

Cyanmethemoglobin method (HICN)

Question 8

Cyanmethemoglobin method (HICN) is recommended because it measures all forms of Hb except ________

Answer 8

Sulfhemoglobin

Question 9

Cyanmethemoglobin method (HICN)
spx:

Answer 9

20 uL blood

Question 10

Cyanmethemoglobin method (HICN)
reagent:

Answer 10

5 mL Drabkin’s solution TF

Question 11

responsible for converting hemoglobin to
hemiglobin

Answer 11

Potassium ferricyanide

Question 12

for converting hemiglobin to hemiglobin cyanide

Answer 12

Potassium cyanide

Question 13

accelerator for Cyanmethemoglobin method (HICN)

Answer 13

Dihydrogen potassium phosphate

Question 14

Use ______ reagent as blank in HICN

Answer 14

cyanmethemoglbin

Question 15

Cyanmethemoglobin method (HICN)
product:

Answer 15

HICN measured at 540 nm

Question 16

T or F
Cyanmethemoglobin reagent is sensitive to light

Answer 16

T

Question 17

T or F
in HICN, turbidity of sample can cause false-decrease values

Answer 17

F
falsely-increase

Question 18

HICN
Remedy:
High WBC count
High PLT count

Answer 18

Centrifuge reagent-sample mixture and
measure the supernatant

Question 19

HICN
Remedy:
Lipemia

Answer 19

Mix 0.01 mL of patient’s plasma + 5 mL
of cyanmethemoglobin reagent, and use
it as reagent blank

Question 20

HICN
Remedy:
Hb S and Hb C f:
Prepare ____ dilution with _________
and multiply the result by 2

Answer 20

1:2
distilled water

Question 21

T or F
Hb S is insoluble in low O2 tension

Answer 21

T

Question 22

causes crystal formation

Answer 22

Hb C

Question 23

Indirect method of Hemoglobin determination:
Chemical: (Principle: 1 gHb contains _______ )
Gasometric (Principle: 1 g Hb contains _______ )

Answer 23

3.47 g Fe
1.34 mL 02

Question 24

Gravimetric:
Specific gravity: (CuSO4 method: sg. of _____)

Answer 24

1.053