M2: DNA Transcription Flashcards
Process by which the information in a strand of DNA is copied into a new molecule of RNA
Transcription
Regulated process of DNA Transcription
- Protein-demand dependent
- Conservation of Cell Resources
- Enhanced Cell Fitness
Evidences of RNA as Intermediate Molecule in the Genetic Information Flow
- DNA does not participate in protein synthesis
- RNA is synthesized in nucleus or nuclear region
- RNA migrates to cytoplasm for protein synthesis
- RNA is chemically similar to DNA
DNA serving as a template for RNA Polymerase
DNA Template Strand
Similar 5’ to 3’ with transcribed RNA molecule
DNA Coding Strand
Initial step of Transcription
Template Binding
Recognized by sigma factors located upstream in the gene that signals the start of initial binding
Promoter
Cluster of gene found in bacteria that regulates genes
Operons
Similar sequences
- Different genes in the same organism
- One or more genes of related organisms
Promoters of Bacteria
Not similar promoter to Eukarya
Promoters of Archaea
Similar promoter to Eukarya
Recognition Sequences in Eukaryotic Promoter Region
- TATA - Goldberg-Hoyness Box
- CAAT/CCAAT box
- GC
Cis-Acting DNA Elements in Eukaryotes
- Core Promoter
- Proximal Promoter
- Enhancers and Silencers
Determines the binding site of RNAP II
Core Promoter
Helps modulate the level of transcription
Proximal Promoter
Cis-acting element that influence the efficiency or rate of transcription initiation by RNAP II
Enhancers and Silencers
Facilitates RNAP II binding and initiation of transcription
Trans-Acting Factors
Required trans-acting factor for RNAP II mediated transcription
General Transcription Factors
Trans-acting factor that influence the efficiency of RNAP II transcription initiation
Transcriptional activators and repressors
Catalyzed polymerization of RNA molecules, 5’ to 3’
RNA Polymerase