M2: Chemical Basis of Heredity Flashcards
Characteristics of a Genetic Material
- Replication
- Storage of Information
- Variation by Mutation
Direct Proof that DNA is a Genetic Material
- Avery-Macleod-McCarty Experiment
- DNA causes a transformation in bacteria - Hershey-Chase Experiment
- DNA directs the reproduction of viruses
Indirect Proof that DNA is a Genetic Material
- Griffith’s Transforming Principle
- Recombinant DNA Technology
- Phage DNA-mediated infection
- DNA absorbance wavelength
- DNA found on organelles
Building blocks of DNA
2-deoxyribonucleotide
Building blocks of RNA
ribonucleotide
Bond between phosphate and pentose sugar
Phosphoester bond
Bond between base and pentose sugar
n-glycosidic bond
Difference between 2-deoxyribose and ribose
presence of -OH group in 2’ of pentose sugar in Ribose
Pyrimidine
Single rings like Thymine, Uracil and Cytosine
Purine
Double rings like Adenine and Guanine
Bond between 2 nucleotide
Phosphodiester bond
Basis of Watson and Crick DNA model
B DNA
Suggested that DNA is similar to a helix
X-ray Diffraction Data
Position of nitrogenous bases
Nitrogenous bases are planar, perpendicular to the axis, and stacked on one another
Diameter of DNA
20 A or 2.0 nm
Covalent bonds in DNA
bases and sugar (phosphodiester bond)