lineweaver blot Flashcards
1
Q
What is the X-axis
A
1/{S]
2
Q
What is the Y axis
A
1/v
3
Q
what is the x intercept
A
1/-Km
4
Q
What is the Y intercept
A
1/Vmax
5
Q
what is the slope
A
km/vmax
6
Q
What is seen during competitive inhibition
A
crossing of lines and an increase in Km
7
Q
What is seen in non-competitive inhabitation
A
The line gets more vertical you see a decrease in Vmax
8
Q
What do you see in mixed inhabition
A
You see both an increase in Km and a decresein Vmax
9
Q
What do you see in allosteric activation
A
You see a reduction in Km
10
Q
What do you see in co-valent activation
A
you see a increase in Vmax but no change in Km
