lecture 11/12 Flashcards
Within the first step of Glycolysis, what is happening?
Glucose reacts with ATP to form
- ADP
- Glucose-6-phosphate
What happens during the second step of glycolysis?
Glucose-6-phosphate reacts to form fructose-6-phosphate
What happens during the third step of glycolysis?
Fructose-6-phosphate reacts with ATP to form:
- ADP
- Fructose-1,6-diphosphate
What is the key reactant within the first three stages of glycolysis?
ATP
Is oxygen being used within the first three stages of glycolysis?
No, not at all.
What happens at the fourth step of glycolysis?
Fructose-1,6-diphosphate reacts to form one of two things.
- Dihydroxy-acetone-phosphate.
or - Glyceraldehyde-3-phosphate
What does (2) 1,3-Diphosphoglyeric acid react with to make (2) 3-phosphoglyceric acid?
2x ADP
resulting in the production of 2x ATP
Within the secound half of glycolysis, how much ATP is being produced?
4 molecules of ATP
How much ATP is used within the first stage of glycolysis?
2 molecules of ATP
What is the end product of Glycolysis?
2x Pyruvic acid.
What is Pyruvic acid used for?
It provides energy to the cells through the Krebs cycle (TCA cycle)
What happens to Pyruvic acid within the TCA when oxygen is not present?
It ferments and becomes lactic acid.
Where does the TCA take place?
Within the inter membrane space of mitochondria.
What does the TCA cycle interact with to create FADH2
It interacts with inter-membrane protein II to facilitate the production of FADH2.
What is the goal of glycolysis?
To transform glucose into pryuvate, so it can entre into the Krebs cycle to produce more energy and produce more ATP in the process.
Within the Electron Transport Chain, how many proton pumps are there? How many carriers?
3 pronton pumps
2 carriers
What are the numerals for the proton pumps?
I,III, and IV are all proton pumps.
What is the goal of the proton pumps within the ETC?
They move ions across their gradient into the inner membrane space
What is used to facilitate the movement of ions through the ETC proton pumps?
NADH2 is used.
it gives up a proton and electrons, oxidizing to become NAD+
What is the function of Ubiquinone within the ETC?
It acts as a carrier of protons moving them to complex III
What is the function of Cytochrome C within the ETC?
It acts as a proton carrier and moves them to proton pump IV
What is the byproduct of the ETC?
It produces Water.
and it produces ATP
What is the main purpose of the ETC?
to generate a proton gradient.
What is an adaptation of mitochondria that allows for greater volume of ETC function?
The Mitochondria has a folded matrix, greatly increasing its SA/V ration.
What is the function of Complex 1?
It accepts hydrogen from NADH2, thereby regenerating NAD+.
The complex becomes reoxidized by passing hydrogen to Ubiquinone.
What is the function of Complex II?
It oxidizes succinate in the Kreb’s cycle, passing hydrogen’s to FAD to form FADH2, which then passes the Hydrogen’s to Ubiquinone.
What is the function of complex IV?
Passes electrons to elemental oxygen, forming water.
What happens as Electrons flow through complexes I,III, and IV?
They lose energy, which is then used to pump protons from the mitchondrial matrix into the inter membrane space.
What do you need to generate ATP? ___+___=ATP?
ADP + Pi