Lesson 9 Part 2 Flashcards
The proliferation or multiplication of becateria
Bacterial growth
Bacteria multiply by _____
Binary fission
The time it takes a particular bacterial species to undergo binary fission
Generation time
Bacteria with short generation time
Rapid growers
Bacteria with long generation time
Slow growers
Microorganisms that are difficult to grow in the laboratory
Fastidious
The media that are used in microbiology laboratories to culture bacteria are called
Artificial media or synthetic media
All the ingredients are known; the medium was prepared in the laboratory
Chemically defined medium
The exact contents are not known; contain ground up or digested extracts from animal organs and plants
Complex medium
They are also known as broth
Liquid media
Contained in tubes and are thus often referred to as tube media
Liquid media
Prepared by adding agar to liquid media and then pouring the media into tubes or petrid dish
Solid media
Complex polysaccharide that is obtained from red marine algae
Agar
A broth or solid medium containing a rich supply of special nutrients
Enriched medium
Used to discourage the growth of certain organism
Selective medium
Inhibits growth of gram POSITIVE bacteria
MacConkey agar
Inhibits growth of gram negstivr
Phenylethyl alcohol (PEA) and Colistinnalidixic acid (CNA)
Inhibits growth of gram negative
Phenylethyl alcohol agar (PEA) and collistinnalidixic acid (CNA)
Chocolate agars; selective for N. Gonorrheae
Thayer-Martin agar and Martin Lewis agar
Grows only salt tolerant (haloduric) bacteria
Mannitol salt agar (MSA)
Allows one to readily differentiate among the various types of organisms that are growing on the medium
Differentiate Medium
It differentiate between lactose-fermenting and non-lactose fermenting gram negative bacteria
MacConkey Agar
It used to screen s aureus
Mannitol Salt Agar
Ueed to determine the type of hemolysis
Blood agar
A very popular liquid medium for use in the bacteriology laboratory
Thioglycollate broth (THIO)
It is practised in the microbiology laboratory to prevent infection of individuals and contamination of the work environment, clinical specimens, and culture
Aseptic Technique
After media is inoculated, the next step is
Incubation
It is placed in a chamber that contains the appropriate atmosphere and moisture level and is set to maintain the appropriate temperature
Incubation
To culture most human pathogens, the incubator is set at ______ to _____ C
35°C to 37°C
What are the three types of incubators
CO2 incubators
Non-CO2 incubators
Anaerobic incubators
Involves the destruction or elimination of all microbes, including cells, spores, and viruses
Sterilization
Describes the elimination of most or all pathogens (except bacterial spores) from non living objects
Disinfection
A method of disinfecting liquids; used to eliminate pathogens from milk and most other beverages
Pasteurization
Chemicals used to disinfect inanimate objects, such as bedside equipment and operating rooms
Disinfectants
Solutions used to disinfect skin and other living tissues
Antiseptic
The reduction of microbial populations to levels considered safe by public health standards, such as restaurants
Sanitization
-cide or -cidal means
killing
Example of disinfectants or antiseptics to kill microbes
Germicides
Biocides
Microbisides
They specifically kill bacteria
Bactericides / bactericidal agents
Required to kill bacterial endospores
Sporicidal agent
Kill fungi, including fungal spores
Fungicidal agents
Are used to kill algae in swimming pools and hot tubs
Algicidal agents
Destroy viruses
Virucidal agents
Kill pseudomonas species
Pseudomonicidal agents
Kill M. Tuberculosis
Tuberculocidal Agents
