Lecture 1 Introduction

Question 1

In the word histology, histo comes from greek which means?

Answer 1

web or tissue

Question 2

it requires the use of “microscopes” to view the structures under increasing magnifications. this requires preparation of slides that will then be viewed under microscopes.

Answer 2

histology

Question 3

How to prepare a slide? what are the steps?

Answer 3

Fixation - Dehydration - Embedding - Sectioning - Mounting - Staining - Observation

Question 4

solution is placed in increasing concentrations of alcohol beginning with 50% to 100% .

Answer 4

Dehydration

Question 5

this involves removing the alcohol and replacing it with a chemical that is miscible in both alcohol and paraffin.

Answer 5

Clearing

Question 6

is the process in which excess paraffin is removed from the block to expose the tissue to be cut by the microtome.

Answer 6

trimming

Question 7

involves using a machine called a microtome that cuts sections very thinly in the form of ribbons.

Answer 7

sectioning

Question 8

the thin sections obtained from the microtome are mounted upon glass slides.

Answer 8

mounting

Question 9

the most common stains are hematoxylin and eosin

Answer 9

staining