Introduction to Veterinary Histology Flashcards
is concerned with the external and internal structure of the organism.
Anatomy
encompasses all those structural features studied by direct visual inspection, palpation, and dissection.
Gross Anatomy
is the study of structures that includes minute parts that can be not be seen by naked eye.
Microscopic Anatomy (Histology)
is an integral component of medical education, giving insights into normal structure and function at the microscopic level of the organization.
Histology
the study of microanatomy of cells tussues and organs as seen through the microscope. and it examines the correlation between structure and function
histology
are the building blocks upon which the organs are constructed in logical orderly, and consistent ways.
tissues
the four basic tissue types;
- epithelial tissue
- connective tissue
- muscular tissue
- nervous tissue
•adipose tissue
permits direct observation of living cells.
it is routine because of the use of antibiotics
cell culture
for preparing samples is simple and reliable, although cell and tissue modification results
paraffin technique
is used on histological specimens the primary purpose of stopping postmortem autolysis.
chemical fixation
examples of chemical fixatives;
- formaldehyde
- glutaraldehyde
- paraformaldehyde
- ethyl alcohol
- acetic acid
- mercuric chloride
- picric acid
- potassium dichromate
- chromic acid
- osmic acid
dehydrating agents:
- ethanol- the most common
- butanol
- dioxane
- isopropanol
a concern for the altered morphology and function resulting from fixation and subsequent processing.
freezing techniques
is usually applied to studies conducted with light microscopy
histochemistry
is applicable to electron microscopy
cytochemistry
this technique is useful with light and electron microscopic investigation for identifying and localizing potentially antigenic substances.
immunocytochemistry
the preparation of biological materials for electron microscopy involves procedures similar to those described for the paraffin technique.
transmission electron microscopy
this is used in histology and has a resolution limit of about 2200.
bright-field microscope
this type of microscope uses an ultraviolet radiation source emitting wavelengths between 1000-3000 angstrom.
ultraviolet microscope
this is visible light microscope uses an ultraviolet light source.
fluorescence microscope
this is a modified bright-field microscope. the usual condenser is replaced by one that causes light to strike the image oblique angle.
this is a modified bright-field microscope. the usual condenser is replaced by one that causes light to strike the image oblique angle.
dark-field microscope
