LATG Ch 10 Genetic engineering

Question 1

pronuclear injection of DNA

Answer 1

then the fertilized eggs are harvested from the oviducts of euthanized females the morning after hCG injection and mating

Question 2

cryopreserving embryos

Answer 2

either two-cell- he day after hCG injection and mating and then allowing the embryos to culture overnight, or eight- cell stage-morning of the second day after mating

Question 3

in vitro fertilization

Answer 3

he timing is completely altered to take into account the time at which the egg is most amenable to fertilization—typically between 12 and 17 hours after hCG injection. eggs are harvested early the following morning and placed into drops of swimming sperm for fertilization.

Question 4

Pronuclear injection introduces the transposon-flanked DNA sequence along with an enzyme called a transposase that inserts a single copy of the DNA into the genome.

Answer 4

true

Question 5

If a transgene is integrated in one of the chromosomes of a pair, the animal is said to be:

Answer 5

hemizygous

Question 6

o test for germline transmission, chimeras are mated to animals of the same strain as the blastocyst host.

Answer 6

false

Question 7

Embryonic stem cells used for gene-targeted mutations are grown in the presence of antibiotics.

Answer 7

true

Question 8

An animal developed from a blastocyst injected with embryonic stem cells is known as a

Answer 8

Chimera

Question 9

mosaic (or mosaicism) means

Answer 9

the individual has two or more genetically different cell lines derived from a single zygote.

Question 10

Pronuclei

Answer 10

The nuclei of the eggs shortly after fertilization