CH 9 Molec Biology Flashcards
Mole Biology
Diploid cells:
These cells have chromosomes that are paired. Diploid cells are commonly known as somatic cells and make up all tissues throughout the body.
Haploid cells:
These cells have single chromosomes that are not paired. The only haploid cells are the ovum and spermatid
DNA, each nucleotide consists
a sugar (deoxyribose),
a nitrogen-containing base attached to the sugar, and
a phosphate group.
nucleotides in DNA
adenine (A), guanine (G), cytosine (C), and thymine (T). phosphate group–sugar–phosphate group–sugar– is referred to as the backbone of DNA
nitrogenous bases
rungs of the ladder, with the base of the nucleotide on one strand forming a bond with the base of the nucleotide of the other strand
polymerase chain reaction (PCR) is used to:
clone DNA
polymerase chain reaction (PCR) is used to:
clone DNA
western blot
Proteins are separated by size using gel electrophoresis, then transferred to a membrane and probed with specific antibodies to visualize them.
procedures determines the level of mRNA transcripts in a sample
quantitative RT-PCR
weakest bonds in DNA
base-base