Lab Midterm Flashcards
Implantation occurs
Maintain pregnancy within uterus environment
Progesterone control
What happens to a frog’s car when it breaks down
It gets toad awY
What do rabbits eat for breakfast?
IHOP
Which species has caruncles?
Cow
Ovary shape of cow
Almond/triangle
Ovary shape of sow
Bundle of grapes
Area for placenta attachment
Provides base between mother and fetus
Caruncles
Responsible for follicle development
Ovary
Follicles produce
Progesterone
Only the ______ has more blood flow than the ovary
Brain
Where the urine enters the vagina from the bladder
Suburethral diverticulum
Open cervix: cow
Estrogen control
Produces thin, watery mucus
Closed cervix: cow
Progesterone control
Mucus turns to gel
Provides strong, rhythmic contractions under estrogen control
Propels sperm to site of fertilization
Uterus
Site of fertilization for cow
Oviduct
Species that has point of bifurcation
Sow
Species with a long cervix
Sow
Inside our species
Mare
Follicles on inside, vasculature on outside of ovaries
Species with short cervix
Long cranial vagina, short caudal vagina
Bitch
Species that has placental scars
Queen
Corpus luteum appearance
Yellow body
Corpus luteum produces
Progesterone to maintain pregnance
Corpus albicans appearance
White body
CH appearance
Bloody body
Larger follicles means
More estrogen producex
Ampulla appearance
More folds
More ciliated columnar cells
Thinner muscularis layer
Wider diameter
Bicornuate species
Mare, cow, bitch, queen, sow
Cow uterine horns
Moderately developed
Made uterine horns
Poorly developed - short horns
Mare uterine body
Very large
Species with highly developed uterine horns
Bitch, Queen, sow
Perimetrium location
Outer layer in wall of uterus
Myometrium location
Middle layer in wall of uterus
Endometrium location
Inner layer in wall of uterus
Endometrium is a combination of
Mucosa and submucosa layers
Crypts and folds provide
Areas for sperm to be stored
Species that has 4-5 annular rings
Cow
Species that have a fornix vagina
Mare, ewe, cow
Species that has longitudinal folds
Mare
Species that has a non-functional fornix vagina
Mare
The only place where ovulation can occur on the mare’s ovary
Ovulation fossa
Species that does not have a fornix vagina
Sow
Species that has interdigitating pads
Sow
Site of semen deposition
Fornix vagina
Species with cork screw penis
Boar
Species with urethral process
Ram
Species with penile flap
Dog
Pre-scrotal species
Boar
Post-scrotal species
Ram, Bull
Cow orientation of testis
Vertical
Boar orientation of testis
Horizontal
Ram orientation of testis
Vertical
Dog orientation of testis
Horizontal
Location of sperm production (male gamete)
Produces testosterone
Testis
Controls sigmoid flexure
Retractor penis muscle
Relaxed retractor penis muscle appearance
Straightened
Contracted retractor penis muscle appearance
S-shaped
The sigmoid flexure penis is important for
Erection process
Species with very large vesicular and bulbourethral glands
Boar
Dog accessory glands
No ampulla
No sigmoid flexure
Only prostate gland
Engorged with blood
Important in mating process (dog)
Bulbus glandis
Does a stallion have a sigmoid flexure?
No
How many prostate glands does a Tom have?
2
Tom: Spines of glans penis
Penile spines
Cat is an _________ ovulation
Induced
Helps with thermal regulation of testis
Pampiniform plexus l
Cells: Produces testosterone
Leydig cells
Removes fluids to help concentrate sperm
Sterocilia
Species where temperature stimulates ejaculation
Stallion
2 or more cuboidal layers
Secondary follicle
One layer of squamous cells
Primordial follicles
One layer of cuboidal cells
Primary follicle
What follicle has a basement membrane
Antral follicle
Separates granulosa cells from the Theca interna cells
Basement membrane
Tissue-filled structure
Corpus luteum
Contains frimbria
Ciliated columnar epithelium
Oviduct
Ampulla epithelia
Ciliated columnar
Columnar
Isthmus epithelia
Columnar
Oviduct epithelia
Simple Columnar
Ciliated columnar
Uterus epithelia
Simple columnar
Posterior caudal vagina epithelia
Stratified squamous
Control how and rate of sperm cells developing
Sertoli cells
Head of epididymis epithelia
Pseudostratified columnar
Tall columnar
Classes of cryoprotectants
Penetrating, non-penetrating
Penetrating cryoprotectants at at
Intracellular and extracellular level
Penetrating cryoprotectants examples
Glycerol
DMSO
ethylene glycol
Non-penetrating cryoprotectants act at
Extracellular level
Non-penetrating cryoprotectants examples
PVP, PBA, fructose
Functions of semen extending agents
Provide nutrients —> energy
Protect against rapid cooling
Provide buffer from pH shifts
Maintain osmotic pressure and electrolyte balance
Inhibit bacterial growth
Increase semen volume
Protect sperm during freezing
Semen may be preserved after extension at
Room temp
Slightly below room temp
Cooled at 4 degrees Celsius
Frozen at -196 degrees Celsius
Freezing in the absence of ice
Vitrification
Methods of semen freezing
Vapor freezing
Controlled rate freezing
Vapor freezing
Vapor phase of liquid nitrogen then liquid phase of liquid nitrogen
Controlled rate freezing
Ice crystallization
Describe the process of loading a semen straw for A.I.
- Pull up cyropreservation
- Pull up air
- Pull up sample
- Pull up air
- Pull up more media
- Put end in sealing powder
Agonist
Binds to receptor and elicits same response as native hormone
Agonist examples
hCG
eCG
Estradiol cypionate
Antagonist
Binds to receptor and blocks response
hCG is agonist of
LH
eCG is agonist of
FSH
Estradiol cypionate is agonist of
Estrogen
PG•600 is agonist of
FSH and LH
Minimum volume of sperm concentration for stallions
5 mL
Concentration dosage of sperm
500 million live motile sperm
Mare A.I. process
- Clean genitalia with mild detergent
- Dry
- Observe abrasions
- Inseminate through vagina
Made signs ready to breed
Squatting
Urinating
Winking vulva
Standing to be mounted
Mares with babies
May not exhibit classic estrus signs
Stallion collecting process
- Tease
- Clean penis w/warm water, light pressure
- Retease
- Mount dummy
- Artificial vagina alongside dummy —> tail flicking up/down = ejaculation
- Clean off gel off penis with warm water
Ovulating follicle size
52-53 centimeters
Estradiol testing condition
Uterus is target —> larger uterus
Fluid- filled uterus
PG•600 testing condition
Ovary is target —> larger ovaries
Partially filled uterus
PG•600 secondary effect
Enlarged uterus
This hormone is produced from follicles after stimulation from LH and FSH
Estrogen
This hormone is produced during the luteal phase from the CL
Progesterone
Epididymis body epithelia
Simple cuboidal
Epididymis sterocilia epithelia
Ciliated columnar