Lab Exam Flashcards
What are the factors that can affect efficiency of antibiotics in clinical cases
Site of infection, concentration of antibiotic in tissues, drug pharmacodynamics, host response
What are the limitations of zone of inhibition experiment
Designed for rapid growing bacteria. Ph and moisture etc can affect it
How do you do a gram stain
Crystal violet
Grams iodine
Decolorizer
Safranin
Describe bacterial conjugation
Transmit info through sex pili between pos/Neg bacteria
What is bacterial transduction
Virus takes a portion of bacteria and gives it to another bacteria
What is bacterial transformation
Bacteria dies and DNa free floats and other bacteria pick it up
Do different strains of the Same bacteria look the same ?
Yes
How do you know if conjugation as transfer of dna has occurred ?
If two bacteria are individually resistant to different antibiotics as once mates they grew on plates with the presence of both antibiotics
What is a plaque
Clear areas in agar medium that was previously Seeded with bacteria. Represents the lysis of a phage infected bacterial cell
What are plaque forming units
Used to quantitate the number of infective phage particles in the culture
What stain do you use for yeast
Methylene blue
What is malassezia pachydermatis
The infections of ear and skin of dogs
What does malassezia pachydermatis look like in gram stain
Gram stain: purple and round
What does microsporum canis
Canoe with compartments
What does a microsporum gypseum
Thin cigars with a million of them
