Lab 2 Notes

Question 1

When does DNA replication occur

Answer 1

During Synthesis stage S-phase

Question 2

What are the 6 enzymes used during DNA replication

Answer 2

Helicase
Single Strand Binding Proteins
RNA Primase
DNA Polymerase
RNase H
Ligase

Question 3

What is the function of Helicase

Answer 3

Unwinds the helix

Breaks down the hydrogen bonds to seperate the 2 strands

Question 4

What is the function of Single Strand Binding Proteins

Answer 4

Helps keep the two strands seperate

Question 5

What is the function of RNA Primase

Answer 5

Provides starting location to copy new DNA

Question 6

What is the function of DNA Polymerase

Answer 6

Enzyme that starts at RNA primer and copies DNA
adds nucleotides
can only add nucleotides in a 5’ to 5’ direction

Question 7

What is the Leading strand

Answer 7

piece of DNA that is synthesized continuously

Question 8

What is the Laggin strand

Answer 8

piece of DNA that is built in fragments called Okazaki fragments

Question 9

What is the function of RNase H

Answer 9

removes the RNA primer

Question 10

What is the function of Ligase

Answer 10

Connects Okazaki fragments on lagging strand

Question 11

What does semi-conservative refer to for DNA replication

Answer 11

Refers to the fact that each of the resulting double strands of DNA contains one original strand and one newly synthesized strand

Question 12

What are steps of DNA replication

Answer 12

1. Helicase unwinds the DNA
2. SSBs coat the single strands and prevent reattaching of strands
3. Primase attaches a primer of RNA to template strands
4. Starting at RNA primer DNA polymerase begins creating a new strand in a 5’ to a 3’ direction
5. RNase H removes primer after it’s been used
6. DNA polymerase replaces the removed RNA primers with bases
7. Ligase joins fragments together into one continuous strand