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z_A&P I: BIO425 > Lab 2 > Flashcards

Lab 2 Flashcards

1
Q

Which lens does the user look through on a microscope?

A

Ocular lens

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2
Q

Which lens is used to set various magnifications?

A

Objective lens

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3
Q

Which lens typically is 10x or 15x?

A

Ocular

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4
Q

Which lens has the most varied possible magnification lenses?

A

Objective

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5
Q

Total magnification =

A

Ocular zoom x Objective zoom

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6
Q

If the ocular lens is 10x and the objective lens is set to 4x, what is the total magnification?

A

40x

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7
Q

If the ocular lens is 10x and the objective lens is set to 100x, what is the total magnification?

A

1000x

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8
Q

If the ocular lens is 10x and the objective lens is set to 40x, what is the total magnification?

A

400x

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9
Q

What part of the microscope holds the specimen in place?

A

Specimen stage and clips

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10
Q

What purpose does the stage mover serve on the microscope?

A

To adjust the stage left/right or forward/backward

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11
Q

Why would you use the stage mover?

A

To center the subject in the field of view

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12
Q

How is the nosepiece on the microscope used?

A

Rotate to choose the desired objective lens

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13
Q

What is the light source for a compound microscope?

A

Light bulb

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14
Q

What is the Rheostat used for?

A

To set a brightness for the bulb

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15
Q

What is the Condenser Diaphragm used for?

A

To control the amount of light entering the objective lens.

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16
Q

If you look through the ocular lens and don’t see anything, what items would you check?

A
  • power switch - plug (at outlet and at microscope) - rheostat setting - condenser diaphragm is open - objective lens is locked in place - slide position - if outlet is dead
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17
Q

At which settings are you permitted to use both course and fine adjustment knobs?

A

4x and 10x

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18
Q

At which settings are you use only fine adjustment knobs? Why?

A

40x and 100x. Using the course adjustment could push the objective lens through the slide.

19
Q

After a slide is in place on the stage, what is used to center the image?

A

The stage mover knobs

20
Q

As you zoom in the microscope, would you need to increase or decrease the amount of light?

A

Increase

21
Q

As you zoom out the microscope, which you need to increase or decrease the amount of light?

A

Decrease

22
Q

A microscope’s usefulness depends mainly on what two things?

A

Magnification and Resolution Power

23
Q

The minimum distance between two points at which those points can still be seen as different objects refers to what microscope feature?

A

Resolution Power

24
Q

What does resolution power provide?

A

Greater image detail

25
Q

High zoom + low resolution results in

A

Narrow field of view with blurry to no detail image

26
Q

Low zoom + high resolution results in

A

Wide field of view with sharp image

27
Q

High zoom + high resolution results in

A

Narrow field of view with sharp, detailed image

28
Q

What is seen through the ocular lens is called…

A

the field of view

29
Q

If the subject is upside down and inverted, what is the problem?

A

The subject was placed on the stage upside down.

30
Q

T or F: Specimen size is the same as field of view diameter.

A

False

31
Q

If you move the stage right, the image will move…

A

Left

32
Q

The distance between the nearest and farthest objects that remain in focus is called…

A

Depth of field

33
Q

When zoom increases, field of view

A

Decreases

34
Q

When zoom decreases, field of view

A

Increases

35
Q

If the specimen has disappeared from the field of view, what has happened?

A

Zoom has increased

36
Q

Why do we center the image each time the zoom is changed?

A

To make sure it remains in field of view as zoom settings increase

37
Q

The field of view is measured in

A

mm

38
Q

To determine the width of the field of view, we measure its

A

Diameter

39
Q

When we have an original diameter and magnification settings and are provided either one new diameter or magnification setting, what is the formula to figure out the missing diameter or magnification setting?

A

D1M1 = D2M2

40
Q

D1M1 = D2M2

What is the formula to solve for D2?

A

D2 = D1M1 / M2

41
Q

D1M1 = D2M2

What is the formula to solve for M2?

A

M2 = D1M1 / D2

42
Q

A light microscope that uses multiple lenses for magnification is called a…

A

Compound microscope

43
Q

What is the field of view?

A

What the observer sees when they look through the ocular lens.

44
Q

What happens if you use a t-shirt or kleenex to clean the microscope lenses?

A

It will scratch them

z_A&P I: BIO425 (17 decks)

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