Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Lab 1 Test > Flashcards

Lab 1 Test Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Cultural Characteristics

A

refers to how microorganisms appear on media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Filiform

A

uniform looking slant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Echinulate

A

Toothy uniform slant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Beaded

A

uniform with beads straying at the top

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Effuse

A

spreading clumps

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Arborescent

A

A little tree-like

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Rhizoid

A

very tree like

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Ring

A

Growth on sides but not center, like a donut

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Pellicle

A

thicker growth as a layer on top, if it is thin it is membranous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Membranous

A

growth as a very thin layer on top

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Flocculent

A

Floating clumps on surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Turbid

A

cloudy subsurface growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Granular

A

small discrete particles are seen (like salt in water)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Flocculent

A

floating clumps of bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Flakey

A

large particles (flakes) are seen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Viscid

A

thick and sticky

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Sabouraud agar

A

is good for fungal growth

18
Q

Nutrient agar

A

is good for bacterial growth

19
Q

Basic dyes

A

like methylene blue are positive and thus stick to negatively charged bacteria cell walls.

20
Q

Common Basic Dyes

A

methylene blue, crystal violet, safranin, and malachite green

21
Q

Acidic dyes

A

like eosin, contain negatively charged color-bearing ions and will not stick to bacteria

22
Q

Common Acidic Dyes

A

Indian ink, congo red, nigrosoine, eosin

23
Q

Ocular lens

A

eye piece

24
Q

Body tube

A

in between ocular lens and objective lens

25
Q

Coarse focusing knob

A

more general focus

26
Q

Fine focusing knob

A

more finer focus

27
Q

Objective Lenses

A

primary lens that magnify the specimen

28
Q

Stage

A

holds slide in position

29
Q

Condenser Focuses

A

focuses light through the specimen right below stage

30
Q

Diaphragm

A

controls the amount of light entering the condenser

31
Q

Simple staining

A

only uses one basic stain, procedure consists of taking the slide, drowning it in methylene blue for 1 minute then washing it off and blotting

32
Q

Gram staining

A

separate gram negative and gram positive bacteria, with gram positive being vulnerable to antibiotics
Gram positive turn purple after decoloring and gram negative lose their color
So if you want to see gram negative they are counter stained with safranin to look pink
Heat fix → Crystal violet (both turn purple) → iodine both stay purple (it is used as mordant by fixing the crystal violet onto it)→ Alcohol acetone gram negatives are now colorless → safranin gram-negatives turn pink

33
Q

Acid-fast

A

mainly used for identification of the medically important microorganisms, like he agents that cause tuberculosis and leprosy
Cover smear with carbolfuchsin→ decolorize with acd alcohol → counterstain with methylene blue → blot dry

34
Q

Capsular staining-

A

used to view a gelatinous coat called a CAPSULE that is comprised of glycoproteins or polypeptides. What makes this special is because we use negative staining and stain the background as contrast
nigrosine to stain background → add your sample → crystal violet for the capsule viewing

35
Q

Schaeffer-Fulton Endospore staining

A

used to view endospores
Prepped smear of sample , then place over boiling water while having malachite green , then cool and wash to counterstain with safranin
The spores contain the green while the cells are pink

36
Q

Dorner Endospore staining

A

Add drops of water onto a slide and introduce your sample , add 5 drops of carbolfuchsin and boil for 5 min, then mix onto a slide with nigrosin, use a spreader slide.

37
Q

Peritrichous

A

bunch of tails all over the place

38
Q

Lophotrichous

A

a bunch of flagella coming from one side

39
Q

Monotrichous

A

one flagella

40
Q

Amphitrichous

A

one flagella on each side

Microbiology (19 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions