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CAPS 390 > L1. introduction+Staining+Cell > Flashcards

L1. introduction+Staining+Cell Flashcards

1
Q

What is the diameter of the aorta

A

5cm

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2
Q

What is the cross sectional diameter of a small artery

A

1mm

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3
Q

What is an organ?

A

group of tissues that are assembled into units that serve a broader function

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4
Q

What is the diameter of a RBC?

A

7-8 um

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5
Q

What is the diameter of a typical cell?

A

10um

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6
Q

What is the diameter of a nuclei?

A

3um

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7
Q

What is the resolution for LM?

A

up to 0.25um are visible by LM

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8
Q

What is the resolution for EM?

A

anything less than 0.25um is visible through EM

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9
Q

What is the size of a mitochondria?

A

0.2um to 3um

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10
Q

What is the diameter for individual cytoskeletal filaments?

A

5nm -10nm

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11
Q

what is the diameter of microtubules?

A

25nm

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12
Q

What is the diameter of a DNA helix?

A

2nm

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13
Q

What is the process for histology?

A
  1. fixation
  2. dehydration
  3. embedding
  4. sectioning
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14
Q

What is the difference between the process for LM and EM?

A

LM: embedding in parrafin
sectioning is thick with 5-10um
EM: embedding in plastic/resin
sectioning is thin with 25-100nm

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15
Q

What is the process of fixation?

A

cross-link proteins and macromolecules with aldehydes

maintains tissue architecture for microscopic sectioning

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16
Q

What are the different staining types?

A
  1. histochemical : based on charge low specificity
  2. immunochemical: based on antibodies binding to antigens; high specificity
  3. fluorescent tags: linked to various antibodies, facilitates optical sectioning
17
Q

What pH is eosin and hematoxylin?

A

eosin: pink (acidic)
hemotoxylin: blue ( basic)

18
Q

What does Osmium Tetroxide stain for? which kind of microscopy is it used in?

A

Binds with lipids.

Used in EM

19
Q

What microscopy can optically section an image

A

confocal microscopy : can take 3D images

CAPS 390 (35 decks)

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