Ingham-6: MHC I

Question 1

What does MHC stand for?

Answer 1

Major Histocompatibility Complex

Question 2

Where can MHC I receptors be found?

Answer 2

Surface of all nucleated cells

Question 3

What cytokine can induce higher expressions of MHC I?

Answer 3

IFN-gamma

Question 4

What type peptides does MHC I usu. present?

Answer 4

endogenous

Question 5

What are the 2 chain types that make up MHC I

Answer 5

• alpha chains

- beta-2-microglobulin

Question 6

How big of a peptide does MHC I peptide binding groove bind?

Answer 6

8-10 aa

Question 7

What makes up the MHC I peptide binding domain?

Answer 7

alpha 1 and 2 domains (2 alpha helices and 1 beta sheet)

Question 8

Why is there a limit on the size of peptide bound on the groove?

Answer 8

ends of MHC I groove is closed off

Question 9

Where can MHC II receptors be found?

Answer 9

surface of pAPCs

Question 10

What type of peptides do MHC II usu. present?

Answer 10

exogenous

Question 11

What comprises the MHC II receptor?

Answer 11

alpha and beta chain

Question 12

What makes up the binding cleft of MHC II?

Answer 12

alpha and beta chain (alpha 1 and beta 1 domain)

Question 13

How big are the peptides that bind to the MHC II groove?

Answer 13

13-18 aa

Question 14

What allows MHC II to bind bigger peptides?

Answer 14

the ends of its groove are open

Question 15

What are the 2 main differences that distinguish MHC I and MHC II?

Answer 15

size and source of peptides they bind

Question 16

Where is the Human MHC region located in DNA?

Answer 16

Chromosome 6

Question 17

What are the 3 classical MHC I genes?

Answer 17

HLA-A, B, C

Question 18

What are the 3 classical MHC II genes?

Answer 18

HLA-DP, DQ, DR

Question 19

Is HLA-G classical or non-classical MHC?

Answer 19

non-classical

Question 20

Function of HLA-G

Answer 20

prevents rejection of fetus by mother

Question 21

Function of HLA-DM in MHC II

Answer 21

• nonclassical

- involved in peptide loading onto MHC II

Question 22

3 important characteristics of classical MHC genes

Answer 22

• polygenic
• polymorphic
• co-dominant

Question 23

Where are the polymorphisms located in MHC I?

Answer 23

alpha 1 and 2 domains –> the peptide binding groove

Question 24

Anchor residues

Answer 24

similar aa types at specific positions of peptides

Question 25

Characteristics of anchor residues of peptides for MHC I (x2)

Answer 25

• usu. hydrophobic

- usu. located at C-terminal

Question 26

Characteristics of anchor residues of peptides for MHC II (x2)

Answer 26

• usu. at the centre of peptide

- more variable