Ingham-1: B and T cells, Abs

Question 1

2 branches/parts of the adaptive immune system

Answer 1

Humoral and Cell-mediated

Question 2

Which lymphocyte is responsible for humoral immunity?

Answer 2

B cell

Question 3

Which lymphocyte is responsible for cell-mediated immunity?

Answer 3

T cell

Question 4

What is a B cell antigen?

Answer 4

substance that binds Ab

Question 5

What is a B cell antigen made of?

Answer 5

protein, carbohydrate or DNA

Question 6

What is an epitope?

Answer 6

Portion of Ag recognized by Ab

Question 7

What is polyclonal antisera?

Answer 7

collection of diff. Abs from serum that recognize diff. epitopes of the same Ag

Question 8

What are monoclonal Abs?

Answer 8

Ab derived from a single B cell clone (all recognize the same epitope)

Question 9

What 2 chains make up Ab? How many of each?

Answer 9

• heavy and light

- 2

Question 10

What holds heavy and light chains together?

Answer 10

disulfide bonds

Question 11

How many light chain genes do mice and humans have?

Answer 11

2

Question 12

What are the 2 light chain genes called?

Answer 12

kappa and alpha

Question 13

How many light chain alleles do mice and humans have?

Answer 13

4

Question 14

How many heavy chain genes do mice and humans have?

Answer 14

1

Question 15

How many heavy chain alleles do mice and humans have?

Answer 15

2

Question 16

What are the 5 isotypes of heavy chains?

Answer 16

IgA, IgD, IgD, IgE, and IgM

Question 17

What regions of the Ab bind Ag?

Answer 17

variable regions of heavy and light chains

Question 18

Which chains have constant regions (heavy or light)?

Answer 18

both

Question 19

What is the function heavy chain C-terminal constant region?

Answer 19

effector functions

Question 20

What regions of the chains form Ig domain fold?

Answer 20

VH/VL and CH/CL (the ones that are covalently joined together)

Question 21

What is the structure of Ig fold?

Answer 21

2 beta sheets forming a beta sandwich

Question 22

What does CDR stand for?

Answer 22

complementary determining region

Question 23

In terms of CDR, what forms the Ag-binding site? (for BCR)

Answer 23

CDRs of heavy and light chains

Question 24

For TCR, what forms Ag-binding site (in terms of CDR)?

Answer 24

CDRs of alpha and beta chains

Question 25

What loops in VH and VL connect beta strands?

Answer 25

CDRs

Question 26

What does a modular receptor mean?

Answer 26

Has a signalling subunit and an Ag-binding subunit

Question 27

What does ITAM stand for?

Answer 27

Immunoreceptor Tyrosine-Based Activation Motif

Question 28

What is alpha chain equivalent in BCR?

Answer 28

light chain

Question 29

What is beta chain equivalent in BCR?

Answer 29

heavy chain

Question 30

What is signalling subunit of TCR?

Answer 30

CD3

Question 31

Isotypes differ with respect to ___ and ___ of Ig domain

Answer 31

sequence, number

Question 32

Different isotype Fc (constant) region have different ___

Answer 32

functionalities

Question 33

Which isotype activates classical complement pathway?

Answer 33

IgG and IgM

Question 34

Which isotype crosses placenta?

Answer 34

IgG

Question 35

Which isotype binds to Fc receptors of phagocytes?

Answer 35

All except IgD and IgE

Question 36

Which isotype performs mucosal transport via poly-Ig receptor?

Answer 36

IgA, IgM

Question 37

Which isotype induces mast cell/basophil degranulation?

Answer 37

IgE

Question 38

Which isotypes are present on membrane of mature naive B cells?

Answer 38

IgM and IgD