IMMUNOHISTOCHEMISTRY Flashcards
1
Q
identification of specific or selective tissue or cellular antigens found on tissues
A
immunohistochemistry
2
Q
most commonly used antibody class in IHC
A
IgG
3
Q
most widely used enzyme labels
A
Horse Radish Peroxidase (HRP)
4
Q
most common chromogen; used for most applications and provides strong and permanent stains
A
DAB (Diaminobenzidine) (dark brown)
5
Q
employed for skin sections
A
AP Red (DAP can be masked by melanin pigment)
6
Q
most cost effective label
A
enzyme labels
7
Q
done prior to processing; prerequisite especially for paraffin embedded tissues
A
unmasking of Ag/Ag retrieval
8
Q
methods to retrieve antigens (Ag unmasking) from Routinely Processed Tissue
A
- Proteolytic Enzyme Retrieval (use of pepsin and bromelin, trypsin, protease)
- Microwave Antigen Retrieval (Heat Induced Epitope Retrieval)
- Pressure Cooking Ag retrieval
- Autoclave heating
- Waterbath heating (90C; better retrieval at 95-98C)
- Steamer Heating
- Decloaker Heating
- Combination of Microwave and Enzyme digestion
