CLEARING Flashcards
aka alcoholization
clearing
removal of the dehydrating agent and replacement of a fluid (clearing agent) that is miscible with both the dehydrating agent impregnating/embedding medium
clearing
makes the tissues “translucent” (increases refractive index)
clearing
over clearing may cause ____
tissue brittleness
commonly used clearing agents
- xylene
- toulene
- benzene
- chloroform
- cedarwood oil
- aniline oil
- clove oil
- carbon tetrachloride
xylene aka
xylol
most commonly used clearing agent in routine procedures
xylene
milky xylene is due to
incomplete dehydration
substitute for xylene or benzene; toxic upon prolonged exposure
toluene (toluol)
can cause aplastic anemia (pancytopenia) and carcinogenic
benzene
recommended for tough tissues, brain, lymph nodes
chloroform
tissues tend to _____ in chloroform
float (anong ginagawa sa mga lutang? balutan ng gauze)
hepatotoxic
chloroform
recommended for CNS tissues and cytology such as smooth muscles and skin; very slow and expensive
cedarwood oil