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HISTOPATH > DEHYDRATION > Flashcards

DEHYDRATION Flashcards

1
Q

removal of intracellular and extracellular water from tissues

A

dehydration

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2
Q

amount of dehydrating agent

A

at least 10x that of the tissue

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3
Q

commonly used dehydrating agents

A
  • alcohol
  • acetone
  • dioxane
  • cellosolve
  • triethyl phosphate
  • tetrahydrofuran (THF)
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4
Q

recommended for routine tissue dehydration

A

ethyl alcohol (ethanol)

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5
Q

best dehydrating agent

A

ethyl alcohol (Ethanol)

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6
Q

fast-acting, mixes with water and many inorganic solvents, penetrates tissues easily, not poisonous and expensive

A

ethyl alcohol

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7
Q

primarily used for blood and tissue films; toxic when ingested

A

methanol

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8
Q

utilized in plant animal microtechnique; slow acting but there is less shrinkage

A

butanol

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9
Q

univeral solvent. dehydrates and clears

A

tertiary butanol (82%)

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10
Q

dissolves paraffin

A

pentanol

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11
Q

excellent substitute for ethanol

A

isopropanol

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12
Q

best clearing agent for microwave technique

A

isopropanol

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13
Q

insures complete dehydration

A

anhydrous copper sulfate

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14
Q

indicates presence of water

A

blue

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15
Q

clear, colorless liquid, highly miscible but flammable

A

acetone

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16
Q

cheap, rapid acting

17
Q

not recommended; lumiliit and rumurupok tissue

18
Q

for most urgent biopsies (1/2 to 2 hrs) but can cause considerable amount of shrinkage

19
Q

aka diethylene dioxide

20
Q

serves as both a dehydrating and clearing agent

21
Q

produces less tissue shrinkage; pero pangit tissue ribbon

22
Q

disadvantages of dioxane

A
  • expensive
  • tissues tend to ribbon poorly
  • highly toxic
23
Q

uses pure dioxane and paraffin

A

graupner’s

24
Q

tissue is wrapped in gauze and suspended in a bottle containing dioxane and anhydrous calcium oxide/quicklime

A

Weiseberger’s

25
Q

clearing agent na amoy rotten egg

A

carbon disulfide

26
Q

aka Ethylene Glycol Monoethyl Ether

A

cellosolve

27
Q

rapid dehydrating agent

A

cellosolve

28
Q

combustible at 110 - 120F and toxic

A

cellosolve

29
Q

removes water very readily and produces very little distortion and hardening; hydrate sections and smears following certain stains

A

Tri-Ethyl Phosphate

30
Q

dehydrating and clearing agent

A

tetrahydrofuran

31
Q

toxic if ingested or inhaled; has an offensive odor

A

tetrahydrofuran

32
Q

can cause conjunctival irritation that may lead to blindness

A

tetrahydrofuran

33
Q

universal solvents: dehydrate and clear at the same time

A

tertiary butanol. tetrahydrofuran, dioxane

34
Q

dehydrating agents for EM

A

ethanol, propylene oxide, acetonitirile

HISTOPATH (17 decks)

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